Effect of oral fat on plasma levels of neurotensin and neurotensin fragments in humans - Characterization by high-pressure liquid chromatography

Edwin J. Draviam; James R. Upp; George H. Greeley; Courtney M. Townsend; James C. Thompson

doi:10.1007/BF01536763

Effect of oral fat on plasma levels of neurotensin and neurotensin fragments in humans - Characterization by high-pressure liquid chromatography

Edwin J. Draviam, James R. Upp, George H. Greeley, Courtney M. Townsend, James C. Thompson

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The effect of ingestion of fat (Lipomul 1 g/kg) on the circulating levels of neurotensin (NT^1-3) and amino-terminal fragments (NT^1-8, NT^1-11) and carboxy-terminal fragment (NT^8-13) of NT were investigated in six healthy male volunteers. NT and NT fragments were extracted from plasma collected at 0, 15, 30, and 60 min after ingestion of fat, and the plasma levels of NT^1-13 and NT fragments were characterized using high-pressure liquid chromatography and radioimmunoassay techniques. Significant elevations of plasma levels of NT^1-8, NT^1-11, and NT^1-13 were observed at 15, 30, and 60 min after fat ingestion. The maximum elevations were 273% for NT^1-8, 234% for NT^1-11, and 54% for NT^1-13. NT^8-13 levels failed to rise significantly when compared to basal levels. These findings indicate that both the aminoterminal and carboxyterminal fragments of NT are either released along with intact NT or are formed as metabolites from NT^1-13 in response to ingestion of fat in man.

Original language	English (US)
Pages (from-to)	200-204
Number of pages	5
Journal	Digestive Diseases and Sciences
Volume	35
Issue number	2
DOIs	https://doi.org/10.1007/BF01536763
State	Published - Feb 1990
Externally published	Yes

Keywords

high-pressure liquid chromatography
neurotensin
peptide
radioimmunoassay

ASJC Scopus subject areas

Physiology
Gastroenterology

Access to Document

10.1007/BF01536763

Cite this

@article{479a0b069019446a869c2f45c8b65e40,

title = "Effect of oral fat on plasma levels of neurotensin and neurotensin fragments in humans - Characterization by high-pressure liquid chromatography",

abstract = "The effect of ingestion of fat (Lipomul 1 g/kg) on the circulating levels of neurotensin (NT1-3) and amino-terminal fragments (NT1-8, NT1-11) and carboxy-terminal fragment (NT8-13) of NT were investigated in six healthy male volunteers. NT and NT fragments were extracted from plasma collected at 0, 15, 30, and 60 min after ingestion of fat, and the plasma levels of NT1-13 and NT fragments were characterized using high-pressure liquid chromatography and radioimmunoassay techniques. Significant elevations of plasma levels of NT1-8, NT1-11, and NT1-13 were observed at 15, 30, and 60 min after fat ingestion. The maximum elevations were 273% for NT1-8, 234% for NT1-11, and 54% for NT1-13. NT8-13 levels failed to rise significantly when compared to basal levels. These findings indicate that both the aminoterminal and carboxyterminal fragments of NT are either released along with intact NT or are formed as metabolites from NT1-13 in response to ingestion of fat in man.",

keywords = "high-pressure liquid chromatography, neurotensin, peptide, radioimmunoassay",

author = "Draviam, {Edwin J.} and Upp, {James R.} and Greeley, {George H.} and Townsend, {Courtney M.} and Thompson, {James C.}",

year = "1990",

month = feb,

doi = "10.1007/BF01536763",

language = "English (US)",

volume = "35",

pages = "200--204",

journal = "Digestive Diseases and Sciences",

issn = "0163-2116",

publisher = "Springer New York",

number = "2",

}

TY - JOUR

T1 - Effect of oral fat on plasma levels of neurotensin and neurotensin fragments in humans - Characterization by high-pressure liquid chromatography

AU - Draviam, Edwin J.

AU - Upp, James R.

AU - Greeley, George H.

AU - Townsend, Courtney M.

AU - Thompson, James C.

PY - 1990/2

Y1 - 1990/2

N2 - The effect of ingestion of fat (Lipomul 1 g/kg) on the circulating levels of neurotensin (NT1-3) and amino-terminal fragments (NT1-8, NT1-11) and carboxy-terminal fragment (NT8-13) of NT were investigated in six healthy male volunteers. NT and NT fragments were extracted from plasma collected at 0, 15, 30, and 60 min after ingestion of fat, and the plasma levels of NT1-13 and NT fragments were characterized using high-pressure liquid chromatography and radioimmunoassay techniques. Significant elevations of plasma levels of NT1-8, NT1-11, and NT1-13 were observed at 15, 30, and 60 min after fat ingestion. The maximum elevations were 273% for NT1-8, 234% for NT1-11, and 54% for NT1-13. NT8-13 levels failed to rise significantly when compared to basal levels. These findings indicate that both the aminoterminal and carboxyterminal fragments of NT are either released along with intact NT or are formed as metabolites from NT1-13 in response to ingestion of fat in man.

AB - The effect of ingestion of fat (Lipomul 1 g/kg) on the circulating levels of neurotensin (NT1-3) and amino-terminal fragments (NT1-8, NT1-11) and carboxy-terminal fragment (NT8-13) of NT were investigated in six healthy male volunteers. NT and NT fragments were extracted from plasma collected at 0, 15, 30, and 60 min after ingestion of fat, and the plasma levels of NT1-13 and NT fragments were characterized using high-pressure liquid chromatography and radioimmunoassay techniques. Significant elevations of plasma levels of NT1-8, NT1-11, and NT1-13 were observed at 15, 30, and 60 min after fat ingestion. The maximum elevations were 273% for NT1-8, 234% for NT1-11, and 54% for NT1-13. NT8-13 levels failed to rise significantly when compared to basal levels. These findings indicate that both the aminoterminal and carboxyterminal fragments of NT are either released along with intact NT or are formed as metabolites from NT1-13 in response to ingestion of fat in man.

KW - high-pressure liquid chromatography

KW - neurotensin

KW - peptide

KW - radioimmunoassay

UR - http://www.scopus.com/inward/record.url?scp=0025181830&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025181830&partnerID=8YFLogxK

U2 - 10.1007/BF01536763

DO - 10.1007/BF01536763

M3 - Article

C2 - 2302977

AN - SCOPUS:0025181830

SN - 0163-2116

VL - 35

SP - 200

EP - 204

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

IS - 2

ER -

Effect of oral fat on plasma levels of neurotensin and neurotensin fragments in humans - Characterization by high-pressure liquid chromatography

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this