Effect of mucosal fluid from women with bacterial vaginosis on HIV trans-infection mediated by dendritic cells

Elizabeth P. St. John, M. Reza Zariffard, Jeffrey A. Martinson, Jose A. Simoes, Alan L. Landay, Gregory T. Spear

Research output: Contribution to journalArticlepeer-review

Abstract

Women with bacterial vaginosis (BV) have a higher risk of HIV transmission but the cause of risk is unknown. Dendritic cells (DC) are implicated in transmission of HIV and we previously observed that DC mature when exposed to mucosal fluid from women with BV. We hypothesized that maturation of DC by BV mucosal fluid would enhance DC-mediated trans-infection of HIV. Monocyte-derived DC (MDDC) were treated with mucosal fluid, incubated with HIVBal, and HIV trans-infection was evaluated. While LPS-treated MDDC increased HIVBal trans-infection, BV fluid reduced trans-infection. HIVBal DNA levels in MDDC were not affected by BV fluid or LPS but productive infection of MDDC was decreased by LPS and BV fluid. Mucosal fluid from women with BV does not increase MDDC-mediated trans-infection suggesting that BV does not increase HIV susceptibility by increasing DC-mediated trans-infection. However, indirect effects of DC maturation on HIV transmission cannot be ruled out.

Original languageEnglish (US)
Pages (from-to)22-27
Number of pages6
JournalVirology
Volume385
Issue number1
DOIs
StatePublished - Mar 1 2009
Externally publishedYes

Keywords

  • Activation
  • Bacterial vaginosis
  • Cervical vaginal lavage
  • Dendritic cells
  • HIV
  • Maturation
  • MDDC
  • Toll-like receptors

ASJC Scopus subject areas

  • Virology

Fingerprint

Dive into the research topics of 'Effect of mucosal fluid from women with bacterial vaginosis on HIV trans-infection mediated by dendritic cells'. Together they form a unique fingerprint.

Cite this