Effect of antibody-mediated neutropenia on the cardiopulmonary response to endotoxemia

J. O. Basadre, H. Singh, D. N. Herndon, J. Stothert, L. D. Traber, K. Horn, K. LeBlanc, J. T. Flynn, D. L. Traber

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


An ovine anti-neutrophil antibody has been produced by immunizing rabbits with purified sheep neutrophils. Serial intraarterial infusions of anti-neutrophil antibody in awake instrumented sheep produced selective and profound neutropenia. Intravascular infusion of endotoxin (Escherichia coli, 1.5 μg/kg/30 min) resulted in significant and equivalent increases in pulmonary artery pressure, peripheral vascular resistance, and protein-rich pulmonary lymph flow in an endotoxin group (n = 9) and a depletion + endotoxin group (n = 4). Changes in cardiopulmonary parameters were most pronounced 2 to 8 hr after endotoxin administration in both groups. Cardiac index (CI) showed a precipitous and transient fall in both experimental groups at 0.5 to 1 hr after endotoxin infusion; however, by 8 hr CI rose significantly in the endotoxin group, while it remained unchanged in the depletion + endotoxin group. A significant rise in the peripheral neutrophil count was associated with the increase in CI in the endotoxin group. Plasma and pulmonary lymph levels of thromboxane -B2 were unchanged during the depletion period with a significant increase 1 hr after endotoxin infusion. In this study questions arise regarding the exclusive role of circulating neutrophils in the microvascular permeability changes seen in sepsis-mediated adult respiratory distress syndrome.

Original languageEnglish (US)
Pages (from-to)266-275
Number of pages10
JournalJournal of Surgical Research
Issue number3
StatePublished - Sep 1988
Externally publishedYes

ASJC Scopus subject areas

  • Surgery


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