Ebola virus infection induces autoimmunity against dsDNA and HSP60

H. Fausther-Bovendo; X. Qiu; S. McCorrister; G. Westmacott; P. Sandstrom; C. Castilletti; A. Di Caro; G. Ippolito; G. P. Kobinger

doi:10.1038/srep42147

Ebola virus infection induces autoimmunity against dsDNA and HSP60

H. Fausther-Bovendo, X. Qiu, S. McCorrister, G. Westmacott, P. Sandstrom, C. Castilletti, A. Di Caro, G. Ippolito, G. P. Kobinger

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Abstract

Ebola virus (EBOV) survivors are affected by a variety of serious illnesses of unknown origin for years after viral clearance from the circulation. Identifying the causes of these persistent illnesses is paramount to develop appropriate therapeutic protocols. In this study, using mouse and non-human primates which survived EBOV challenge, ELISA, western blot, mass spectrometry and flow cytometry were used to screen for autoantibodies, identify their main targets, investigate the mechanism behind their induction and monitor autoantibodies accumulation in various tissues. In infected mice and NHP, polyclonal B cell activation and autoantigens secretion induced autoantibodies against dsDNA and heat shock protein 60 as well as antibody accumulation in tissues associated with long-term clinical manifestations in humans. Finally, the presence of these autoantibodies was confirmed in human EBOV survivors. Overall, this study supports the concept that autoimmunity is a causative parameter that contributes to the various illnesses observed in EBOV survivors.

Original language	English (US)
Article number	42147
Journal	Scientific reports
Volume	7
DOIs	https://doi.org/10.1038/srep42147
State	Published - Feb 9 2017
Externally published	Yes

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title = "Ebola virus infection induces autoimmunity against dsDNA and HSP60",

abstract = "Ebola virus (EBOV) survivors are affected by a variety of serious illnesses of unknown origin for years after viral clearance from the circulation. Identifying the causes of these persistent illnesses is paramount to develop appropriate therapeutic protocols. In this study, using mouse and non-human primates which survived EBOV challenge, ELISA, western blot, mass spectrometry and flow cytometry were used to screen for autoantibodies, identify their main targets, investigate the mechanism behind their induction and monitor autoantibodies accumulation in various tissues. In infected mice and NHP, polyclonal B cell activation and autoantigens secretion induced autoantibodies against dsDNA and heat shock protein 60 as well as antibody accumulation in tissues associated with long-term clinical manifestations in humans. Finally, the presence of these autoantibodies was confirmed in human EBOV survivors. Overall, this study supports the concept that autoimmunity is a causative parameter that contributes to the various illnesses observed in EBOV survivors.",

author = "H. Fausther-Bovendo and X. Qiu and S. McCorrister and G. Westmacott and P. Sandstrom and C. Castilletti and {Di Caro}, A. and G. Ippolito and Kobinger, {G. P.}",

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doi = "10.1038/srep42147",

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T1 - Ebola virus infection induces autoimmunity against dsDNA and HSP60

AU - Fausther-Bovendo, H.

AU - Qiu, X.

AU - McCorrister, S.

AU - Westmacott, G.

AU - Sandstrom, P.

AU - Castilletti, C.

AU - Di Caro, A.

AU - Ippolito, G.

AU - Kobinger, G. P.

PY - 2017/2/9

Y1 - 2017/2/9

N2 - Ebola virus (EBOV) survivors are affected by a variety of serious illnesses of unknown origin for years after viral clearance from the circulation. Identifying the causes of these persistent illnesses is paramount to develop appropriate therapeutic protocols. In this study, using mouse and non-human primates which survived EBOV challenge, ELISA, western blot, mass spectrometry and flow cytometry were used to screen for autoantibodies, identify their main targets, investigate the mechanism behind their induction and monitor autoantibodies accumulation in various tissues. In infected mice and NHP, polyclonal B cell activation and autoantigens secretion induced autoantibodies against dsDNA and heat shock protein 60 as well as antibody accumulation in tissues associated with long-term clinical manifestations in humans. Finally, the presence of these autoantibodies was confirmed in human EBOV survivors. Overall, this study supports the concept that autoimmunity is a causative parameter that contributes to the various illnesses observed in EBOV survivors.

AB - Ebola virus (EBOV) survivors are affected by a variety of serious illnesses of unknown origin for years after viral clearance from the circulation. Identifying the causes of these persistent illnesses is paramount to develop appropriate therapeutic protocols. In this study, using mouse and non-human primates which survived EBOV challenge, ELISA, western blot, mass spectrometry and flow cytometry were used to screen for autoantibodies, identify their main targets, investigate the mechanism behind their induction and monitor autoantibodies accumulation in various tissues. In infected mice and NHP, polyclonal B cell activation and autoantigens secretion induced autoantibodies against dsDNA and heat shock protein 60 as well as antibody accumulation in tissues associated with long-term clinical manifestations in humans. Finally, the presence of these autoantibodies was confirmed in human EBOV survivors. Overall, this study supports the concept that autoimmunity is a causative parameter that contributes to the various illnesses observed in EBOV survivors.

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Ebola virus infection induces autoimmunity against dsDNA and HSP60

Abstract

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