Double isotopic method using dansyl chloride for the determination of GABA in rat C6 astrocytoma cell cultures

R. L. Kohl, W. B. Quay, J. R. Perez‐Polo

Research output: Contribution to journalArticlepeer-review

Abstract

Methods are described for the quantitative measurement of GABA in culture. The method can be adapted to any amino acid or dansyl‐chloride‐reactive species. The sensitivity and selectivity of the procedure result from the double isotopic design in which (14C)‐labeled internal standard was added to the samples before reaction with (3M)‐labeled dansyl chloride. Values obtained by ion‐exchange amino acid analysis of cultures agree closely with the values obtained by the double isotopic method. This method is sensitive enough to measure GABA intracellularly and the condition medium.

Original languageEnglish (US)
Pages (from-to)561-566
Number of pages6
JournalJournal of Neuroscience Research
Volume16
Issue number3
DOIs
StatePublished - 1986
Externally publishedYes

Keywords

  • amino acids
  • glia
  • neurotransmitter

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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