Double-humanized mouse model to study bone morphogenetic protein (BMP) signaling in tumor xenografts

Jenna Newman, Rachel NeMoyer, Andrew Zloza, John Langenfeld

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The activation of the bone morphogenic protein (BMP) signaling pathway in cancer cells has been shown to enhance migration and tumor angiogenesis and promote survival. The BMP signaling pathway regulates benign cells in the tumor microenvironment and is a known regulator of immune cells. The development of BMP receptor inhibitors has allowed the study of tumor xenografts in mice. We describe a double-humanized mouse model with adoptively transferred human immune and human tumor cells that can be used to assess the effects of BMP inhibitors on these human cells in vivo.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages257-262
Number of pages6
DOIs
StatePublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1891
ISSN (Print)1064-3745

Keywords

  • BMP inhibitor
  • Bone morphogenic protein
  • Cell culture
  • Double-humanized mouse model
  • ELISA
  • Flow cytometry
  • Immunohistochemistry
  • NOD scid gamma mice
  • Peripheral blood mononuclear cells
  • Western blotting
  • Xenografts

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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