DNA polymerases: Hoogsteen base-pairing in DNA replication? (reply)

Aneel Aggarwal; Deepak Nair; Robert Johnson; Louise Prakash; Satya Prakash

doi:10.1038/nature04200

DNA polymerases: Hoogsteen base-pairing in DNA replication? (reply)

Aneel Aggarwal, Deepak Nair, Robert Johnson, Louise Prakash, Satya Prakash

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We have solved the crystal structure of human DNA polymerase-? (hPol?) bound to a template primer and incoming nucleotide, and shown that hPol? uses Hoogsteen base-pairing for normal DNA synthesis. Jimin Wang questions the space group we used and claims that Hoogsteen base-pairing is not supported by our electron-density maps. We show here that the issue of space group does not affect our conclusions and that our electron-density maps are clear in indicating that Hoogsteen and not Watson-Crick base-pairing occurs in this situation.

Original language	English (US)
Pages (from-to)	E7
Journal	Nature
Volume	437
Issue number	7057
DOIs	https://doi.org/10.1038/nature04200
State	Published - Sep 14 2005

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title = "DNA polymerases: Hoogsteen base-pairing in DNA replication? (reply)",

abstract = "We have solved the crystal structure of human DNA polymerase-? (hPol?) bound to a template primer and incoming nucleotide, and shown that hPol? uses Hoogsteen base-pairing for normal DNA synthesis. Jimin Wang questions the space group we used and claims that Hoogsteen base-pairing is not supported by our electron-density maps. We show here that the issue of space group does not affect our conclusions and that our electron-density maps are clear in indicating that Hoogsteen and not Watson-Crick base-pairing occurs in this situation.",

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AU - Nair, Deepak

AU - Johnson, Robert

AU - Prakash, Louise

AU - Prakash, Satya

PY - 2005/9/14

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N2 - We have solved the crystal structure of human DNA polymerase-? (hPol?) bound to a template primer and incoming nucleotide, and shown that hPol? uses Hoogsteen base-pairing for normal DNA synthesis. Jimin Wang questions the space group we used and claims that Hoogsteen base-pairing is not supported by our electron-density maps. We show here that the issue of space group does not affect our conclusions and that our electron-density maps are clear in indicating that Hoogsteen and not Watson-Crick base-pairing occurs in this situation.

AB - We have solved the crystal structure of human DNA polymerase-? (hPol?) bound to a template primer and incoming nucleotide, and shown that hPol? uses Hoogsteen base-pairing for normal DNA synthesis. Jimin Wang questions the space group we used and claims that Hoogsteen base-pairing is not supported by our electron-density maps. We show here that the issue of space group does not affect our conclusions and that our electron-density maps are clear in indicating that Hoogsteen and not Watson-Crick base-pairing occurs in this situation.

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DNA polymerases: Hoogsteen base-pairing in DNA replication? (reply)

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