DNA hypomethylation in Morris hepatomas

Lee Jane W. Lu; Erika Randerath; Kurt Randerath

doi:10.1016/0304-3835(83)90159-3

DNA hypomethylation in Morris hepatomas

Lee Jane W. Lu, Erika Randerath, Kurt Randerath

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The 5-methylcytosine (m⁵C) content of DNAs from Morris hepatomas of varying growth rates and from normal liver was analyzed. DNA methylation in all hepatomas studied was found to be 20-45% less than in normal liver. This result was confirmed independently by restriction endonuclease (Hpa II and Msp I) analysis. While these results agreed with recent literature data suggesting hypomethylation of DNA from some neoplastic sources, no correlation was observed between the extent of DNA hypomethylation and the growth rates of the tumors.

Original language	English (US)
Pages (from-to)	231-239
Number of pages	9
Journal	Cancer Letters
Volume	19
Issue number	2
DOIs	https://doi.org/10.1016/0304-3835(83)90159-3
State	Published - Jun 1983
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

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@article{9dec789a45404e10bea8e2b268ad1e93,

title = "DNA hypomethylation in Morris hepatomas",

abstract = "The 5-methylcytosine (m5C) content of DNAs from Morris hepatomas of varying growth rates and from normal liver was analyzed. DNA methylation in all hepatomas studied was found to be 20-45% less than in normal liver. This result was confirmed independently by restriction endonuclease (Hpa II and Msp I) analysis. While these results agreed with recent literature data suggesting hypomethylation of DNA from some neoplastic sources, no correlation was observed between the extent of DNA hypomethylation and the growth rates of the tumors.",

author = "Lu, {Lee Jane W.} and Erika Randerath and Kurt Randerath",

note = "Funding Information: The authors wish to thank Dr. Jean-Numa Lapeyre and Mr. Ted Barna of M.D. Anderson Hospital and Tumor Institute, Houston, Texas, for their help in setting up HPLC analysis for m5C. We are also grateful to Drs. Harris Busch and Lynn C. Yeoman of this Department for permission to use HPLC equipment. We appreciatet he technical assistanceo f Ms. Karen M. Knapp. The support of USPHS Biomedical Research Support grant BRSG-P-122-81 and USPHS grant CA 13591 is gratefully acknowledged.",

year = "1983",

month = jun,

doi = "10.1016/0304-3835(83)90159-3",

language = "English (US)",

volume = "19",

pages = "231--239",

journal = "Cancer Letters",

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TY - JOUR

T1 - DNA hypomethylation in Morris hepatomas

AU - Lu, Lee Jane W.

AU - Randerath, Erika

AU - Randerath, Kurt

N1 - Funding Information: The authors wish to thank Dr. Jean-Numa Lapeyre and Mr. Ted Barna of M.D. Anderson Hospital and Tumor Institute, Houston, Texas, for their help in setting up HPLC analysis for m5C. We are also grateful to Drs. Harris Busch and Lynn C. Yeoman of this Department for permission to use HPLC equipment. We appreciatet he technical assistanceo f Ms. Karen M. Knapp. The support of USPHS Biomedical Research Support grant BRSG-P-122-81 and USPHS grant CA 13591 is gratefully acknowledged.

PY - 1983/6

Y1 - 1983/6

N2 - The 5-methylcytosine (m5C) content of DNAs from Morris hepatomas of varying growth rates and from normal liver was analyzed. DNA methylation in all hepatomas studied was found to be 20-45% less than in normal liver. This result was confirmed independently by restriction endonuclease (Hpa II and Msp I) analysis. While these results agreed with recent literature data suggesting hypomethylation of DNA from some neoplastic sources, no correlation was observed between the extent of DNA hypomethylation and the growth rates of the tumors.

AB - The 5-methylcytosine (m5C) content of DNAs from Morris hepatomas of varying growth rates and from normal liver was analyzed. DNA methylation in all hepatomas studied was found to be 20-45% less than in normal liver. This result was confirmed independently by restriction endonuclease (Hpa II and Msp I) analysis. While these results agreed with recent literature data suggesting hypomethylation of DNA from some neoplastic sources, no correlation was observed between the extent of DNA hypomethylation and the growth rates of the tumors.

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VL - 19

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JO - Cancer Letters

JF - Cancer Letters

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ER -

DNA hypomethylation in Morris hepatomas

Abstract

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