Distribution of Prohormone Convertase-6 Expression in the Gastrointestinal Tract and Effects of a Fasting-Refeeding Regimen and a High-Fat Diet on Ileal Prohormone Convertase-6 Expression

Vidyavathi Udupi; Guillermo Gomez; Isabel Lambertz; Xiang Qi; Jason Reed; George H. Greeley

doi:10.1007/bf02778057

Distribution of Prohormone Convertase-6 Expression in the Gastrointestinal Tract and Effects of a Fasting-Refeeding Regimen and a High-Fat Diet on Ileal Prohormone Convertase-6 Expression

Vidyavathi Udupi, Guillermo Gomez, Isabel Lambertz, Xiang Qi, Jason Reed, George H. Greeley

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Preforms of gastrointestinal peptide hormones and neuropeptides are processed, in part, by prohormone convertases (PCs) to mature, biologically active peptides. The purpose of this study was to characterize the mRNA levels of PC-6 isoforms, PC-6A and PC-6B, in the gastrointestinal tract and pancreas of the rat, and to investigate the effects of a fasting and refeeding regimen, and a high-fat diet on ileal PC-6A expression. PC-6A mRNA is expressed throughout the entire gastrointestinal tract with the highest levels in the small intestine. Multiple-sized transcripts are present. PC-6B mRNA is expressed in the antrum and fundus of the stomach, in the small intestine, and colon. Ileal PC-6A mRNA expression increases significantly with fasting and then declines with refeeding toward control levels. Increased dietary fat increases PC-6A mRNA levels in the ileum. Since PC-6 is found throughout the entire gastrointestinal tract, it is likely that PC-6 participates in the processing of proforms of gastrointestinal peptides. The two isoforms of PC-6 have different patterns of distribution in the gastrointestinal tract and pancreas, suggesting that they process proforms of different gut peptides.

Original language	English (US)
Pages (from-to)	9-13
Number of pages	5
Journal	Endocrine
Volume	7
Issue number	1
DOIs	https://doi.org/10.1007/bf02778057
State	Published - 1997
Externally published	Yes

Keywords

Alimentary tract
Peptide
mRNA

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology

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10.1007/bf02778057

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@article{65c21987645842ad8caade09a7aa73f5,

title = "Distribution of Prohormone Convertase-6 Expression in the Gastrointestinal Tract and Effects of a Fasting-Refeeding Regimen and a High-Fat Diet on Ileal Prohormone Convertase-6 Expression",

abstract = "Preforms of gastrointestinal peptide hormones and neuropeptides are processed, in part, by prohormone convertases (PCs) to mature, biologically active peptides. The purpose of this study was to characterize the mRNA levels of PC-6 isoforms, PC-6A and PC-6B, in the gastrointestinal tract and pancreas of the rat, and to investigate the effects of a fasting and refeeding regimen, and a high-fat diet on ileal PC-6A expression. PC-6A mRNA is expressed throughout the entire gastrointestinal tract with the highest levels in the small intestine. Multiple-sized transcripts are present. PC-6B mRNA is expressed in the antrum and fundus of the stomach, in the small intestine, and colon. Ileal PC-6A mRNA expression increases significantly with fasting and then declines with refeeding toward control levels. Increased dietary fat increases PC-6A mRNA levels in the ileum. Since PC-6 is found throughout the entire gastrointestinal tract, it is likely that PC-6 participates in the processing of proforms of gastrointestinal peptides. The two isoforms of PC-6 have different patterns of distribution in the gastrointestinal tract and pancreas, suggesting that they process proforms of different gut peptides.",

keywords = "Alimentary tract, Peptide, mRNA",

author = "Vidyavathi Udupi and Guillermo Gomez and Isabel Lambertz and Xiang Qi and Jason Reed and Greeley, {George H.}",

note = "Funding Information: The authors acknowledge Karen Martin, Liz Cook, and Eileen Figueroa for their help with the preparation of this article. The authors were supported by funding from NIH grants (PO1 DK35608, RO1 DK15241) and the National Science Foundation (IBN 9506305).",

year = "1997",

doi = "10.1007/bf02778057",

language = "English (US)",

volume = "7",

pages = "9--13",

journal = "Endocrine",

issn = "1355-008X",

publisher = "Humana Press",

number = "1",

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T1 - Distribution of Prohormone Convertase-6 Expression in the Gastrointestinal Tract and Effects of a Fasting-Refeeding Regimen and a High-Fat Diet on Ileal Prohormone Convertase-6 Expression

AU - Udupi, Vidyavathi

AU - Gomez, Guillermo

AU - Lambertz, Isabel

AU - Qi, Xiang

AU - Reed, Jason

AU - Greeley, George H.

N1 - Funding Information: The authors acknowledge Karen Martin, Liz Cook, and Eileen Figueroa for their help with the preparation of this article. The authors were supported by funding from NIH grants (PO1 DK35608, RO1 DK15241) and the National Science Foundation (IBN 9506305).

PY - 1997

Y1 - 1997

N2 - Preforms of gastrointestinal peptide hormones and neuropeptides are processed, in part, by prohormone convertases (PCs) to mature, biologically active peptides. The purpose of this study was to characterize the mRNA levels of PC-6 isoforms, PC-6A and PC-6B, in the gastrointestinal tract and pancreas of the rat, and to investigate the effects of a fasting and refeeding regimen, and a high-fat diet on ileal PC-6A expression. PC-6A mRNA is expressed throughout the entire gastrointestinal tract with the highest levels in the small intestine. Multiple-sized transcripts are present. PC-6B mRNA is expressed in the antrum and fundus of the stomach, in the small intestine, and colon. Ileal PC-6A mRNA expression increases significantly with fasting and then declines with refeeding toward control levels. Increased dietary fat increases PC-6A mRNA levels in the ileum. Since PC-6 is found throughout the entire gastrointestinal tract, it is likely that PC-6 participates in the processing of proforms of gastrointestinal peptides. The two isoforms of PC-6 have different patterns of distribution in the gastrointestinal tract and pancreas, suggesting that they process proforms of different gut peptides.

AB - Preforms of gastrointestinal peptide hormones and neuropeptides are processed, in part, by prohormone convertases (PCs) to mature, biologically active peptides. The purpose of this study was to characterize the mRNA levels of PC-6 isoforms, PC-6A and PC-6B, in the gastrointestinal tract and pancreas of the rat, and to investigate the effects of a fasting and refeeding regimen, and a high-fat diet on ileal PC-6A expression. PC-6A mRNA is expressed throughout the entire gastrointestinal tract with the highest levels in the small intestine. Multiple-sized transcripts are present. PC-6B mRNA is expressed in the antrum and fundus of the stomach, in the small intestine, and colon. Ileal PC-6A mRNA expression increases significantly with fasting and then declines with refeeding toward control levels. Increased dietary fat increases PC-6A mRNA levels in the ileum. Since PC-6 is found throughout the entire gastrointestinal tract, it is likely that PC-6 participates in the processing of proforms of gastrointestinal peptides. The two isoforms of PC-6 have different patterns of distribution in the gastrointestinal tract and pancreas, suggesting that they process proforms of different gut peptides.

KW - Alimentary tract

KW - Peptide

KW - mRNA

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Distribution of Prohormone Convertase-6 Expression in the Gastrointestinal Tract and Effects of a Fasting-Refeeding Regimen and a High-Fat Diet on Ileal Prohormone Convertase-6 Expression

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