Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation

Suxiang Tong; Jairam R. Lingappa; Qi Chen; Bo Shu; Ashley C. LaMonte; Byron T. Cook; Charryse Birge; Wang Chern Shur-Wern; Xin Liu; Renee Galloway; Quynh Mai Le; Fu Ng Wai; Jyh Yuan Yang; Jagdish Butany; James A. Comer; Stephan S. Monroe; Suzanne R. Beard; Thomas G. Ksiazek; Dean Erdman; Paul A. Rota; Mark A. Pallansch; Larry J. Anderson

doi:10.1086/422849

Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation

Suxiang Tong, Jairam R. Lingappa, Qi Chen, Bo Shu, Ashley C. LaMonte, Byron T. Cook, Charryse Birge, Wang Chern Shur-Wern, Xin Liu, Renee Galloway, Quynh Mai Le, Fu Ng Wai, Jyh Yuan Yang, Jagdish Butany, James A. Comer, Stephan S. Monroe, Suzanne R. Beard, Thomas G. Ksiazek, Dean Erdman, Paul A. RotaMark A. Pallansch, Larry J. Anderson

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged, in November 2002, as a novel agent causing severe respiratory illness. To study sequence variation in the SARS-CoV genome, we determined the nucleic acid sequence of the S and N genes directly from clinical specimens from 10 patients-1 specimen with no matched SARS-CoV isolate, from 2 patients; multiple specimens from 3 patients; and matched clinical-specimen/cell-culture-isolate pairs from 6 patients. We identified 3 nucleotide substitutions that were most likely due to natural variation and 2 substitutions that arose after cell-culture passage of the virus. These data demonstrate the overall stability of the S and N genes of SARS-CoV over 3 months during which a minimum of 4 generations for transmission events occurred. These findings are a part of the expanding investigation of the evolution of how this virus adapts to a new host.

Original language	English (US)
Pages (from-to)	1127-1131
Number of pages	5
Journal	Journal of Infectious Diseases
Volume	190
Issue number	6
DOIs	https://doi.org/10.1086/422849
State	Published - Sep 15 2004
Externally published	Yes

ASJC Scopus subject areas

General Medicine

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10.1086/422849

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Tong, S., Lingappa, J. R., Chen, Q., Shu, B., LaMonte, A. C., Cook, B. T., Birge, C., Shur-Wern, W. C., Liu, X., Galloway, R., Le, Q. M., Wai, F. N., Yang, J. Y., Butany, J., Comer, J. A., Monroe, S. S., Beard, S. R., Ksiazek, T. G., Erdman, D., ... Anderson, L. J. (2004). Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation. Journal of Infectious Diseases, 190(6), 1127-1131. https://doi.org/10.1086/422849

Tong, S, Lingappa, JR, Chen, Q, Shu, B, LaMonte, AC, Cook, BT, Birge, C, Shur-Wern, WC, Liu, X, Galloway, R, Le, QM, Wai, FN, Yang, JY, Butany, J, Comer, JA, Monroe, SS, Beard, SR, Ksiazek, TG, Erdman, D, Rota, PA, Pallansch, MA & Anderson, LJ 2004, 'Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation', Journal of Infectious Diseases, vol. 190, no. 6, pp. 1127-1131. https://doi.org/10.1086/422849

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abstract = "Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged, in November 2002, as a novel agent causing severe respiratory illness. To study sequence variation in the SARS-CoV genome, we determined the nucleic acid sequence of the S and N genes directly from clinical specimens from 10 patients-1 specimen with no matched SARS-CoV isolate, from 2 patients; multiple specimens from 3 patients; and matched clinical-specimen/cell-culture-isolate pairs from 6 patients. We identified 3 nucleotide substitutions that were most likely due to natural variation and 2 substitutions that arose after cell-culture passage of the virus. These data demonstrate the overall stability of the S and N genes of SARS-CoV over 3 months during which a minimum of 4 generations for transmission events occurred. These findings are a part of the expanding investigation of the evolution of how this virus adapts to a new host.",

author = "Suxiang Tong and Lingappa, {Jairam R.} and Qi Chen and Bo Shu and LaMonte, {Ashley C.} and Cook, {Byron T.} and Charryse Birge and Shur-Wern, {Wang Chern} and Xin Liu and Renee Galloway and Le, {Quynh Mai} and Wai, {Fu Ng} and Yang, {Jyh Yuan} and Jagdish Butany and Comer, {James A.} and Monroe, {Stephan S.} and Beard, {Suzanne R.} and Ksiazek, {Thomas G.} and Dean Erdman and Rota, {Paul A.} and Pallansch, {Mark A.} and Anderson, {Larry J.}",

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Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation

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