Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation

Suxiang Tong, Jairam R. Lingappa, Qi Chen, Bo Shu, Ashley C. LaMonte, Byron T. Cook, Charryse Birge, Wang Chern Shur-Wern, Xin Liu, Renee Galloway, Quynh Mai Le, Fu Ng Wai, Jyh Yuan Yang, Jagdish Butany, James A. Comer, Stephan S. Monroe, Suzanne R. Beard, Thomas G. Ksiazek, Dean Erdman, Paul A. RotaMark A. Pallansch, Larry J. Anderson

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged, in November 2002, as a novel agent causing severe respiratory illness. To study sequence variation in the SARS-CoV genome, we determined the nucleic acid sequence of the S and N genes directly from clinical specimens from 10 patients-1 specimen with no matched SARS-CoV isolate, from 2 patients; multiple specimens from 3 patients; and matched clinical-specimen/cell-culture-isolate pairs from 6 patients. We identified 3 nucleotide substitutions that were most likely due to natural variation and 2 substitutions that arose after cell-culture passage of the virus. These data demonstrate the overall stability of the S and N genes of SARS-CoV over 3 months during which a minimum of 4 generations for transmission events occurred. These findings are a part of the expanding investigation of the evolution of how this virus adapts to a new host.

Original languageEnglish (US)
Pages (from-to)1127-1131
Number of pages5
JournalJournal of Infectious Diseases
Issue number6
StatePublished - Sep 15 2004
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine


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