Differential surface marker expression in patients with Cd-16+ lymphoproliferative disorders: In vivo model for NK differentiation

Robert A. Bray, Lisa R. Gottschalk, Alan L. Landay, Howard M. Gebel

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we report on three patients, each with a CD-16+ lymphoproliferative disorder. Peripheral blood lymphocyte from all three patients were evaluated for lymphocyte morphology, natural killer (NK) function, and surface marker expression. In addition, two-color flow cytometric analysis was performed to determine the phenotype of the CD-16+ cells. Our findings indicate that the presence of increased numbers of CD-16+ cells alone is not a good predictor of NK activity. However, we observed a differential expression of the HLA class II molecules DR and DQ on the CD-16+ cells obtained from these patients that was associated with NK function. Hence, a CD-16+, Leu-7-, Leu-19+ (NKH-1A) and HLA class II+ phenotype did correlate with NK function in contrast to a CD-16+, Leu-7+, Leu-19- (NKH-1A) and HLA class II-phenotype. Of importance was the fact that the Cd-16+, HLA class II+ cells did not express ED-25 or TFR, nor did they mediate cytotoxicity against solid tumor targets, suggesting that these CD-16+ cells are not activated. Thus, in contrast to previous studies of NK ontogeny that utilized in vitro activated NK cells, studies of patients with CD-16+ lymphoproliferative disorders may provide an alternative aspproach for examining NK differentiation.

Original languageEnglish (US)
Pages (from-to)105-115
Number of pages11
JournalHuman Immunology
Volume19
Issue number2
DOIs
StatePublished - Jun 1987
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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