Differential peroxiredoxin hyperoxidation regulates MAP kinase signaling in human articular chondrocytes

John A. Collins, Scott T. Wood, Jesalyn A. Bolduc, N. P.Dewi Nurmalasari, Susan Chubinskaya, Leslie B. Poole, Cristina M. Furdui, Kimberly J. Nelson, Richard F. Loeser

Research output: Contribution to journalArticlepeer-review

Abstract

The peroxiredoxin (Prx) family of Cys-dependent peroxidases control intracellular levels of H 2 O 2 and can regulate signal transduction. Inhibition of the Prxs, through hyperoxidation amongst other mechanisms, leads to oxidative stress conditions that can alter homeostatic signaling. To determine the effects oxidation of Prx1-Prx3 has on MAP kinase and IGF-1 signaling events in human chondrocytes, this study used 2-methyl-1,4-naphthoquinone (menadione) and 2,3-dimethyl-1,4-naphthoquinone (DMNQ) as H 2 O 2 -generating tools due to their differential mechanisms of action. Menadione and DMNQ generated similar levels of intracellular H 2 O 2 as determined using the biosensor Orp1-roGFP and by measuring Prx redox status. However, menadione generated higher levels of mitochondrial H 2 O 2 associated with Prx3 hyperoxidation and phosphorylation of Prx1 while DMNQ treatment was associated with hyperoxidation of cytosolic Prx1 and Prx2 but not mitochondrial Prx3. Both menadione and DMNQ induced sustained phosphorylation of p38 but only DMNQ activated JNK. Menadione but not DMNQ inhibited IGF-1-induced Akt phosphorylation. Chondrocytes transduced with an adenoviral vector to overexpress Prx3 displayed decreased PrxSO 2/3 formation in response to menadione which was associated with restoration of IGF-1-mediated Akt signaling and inhibition of p38 phosphorylation. Prx1 and Prx2 overexpression had no effects on Prx redox status but Prx1 overexpression enhanced basal Akt phosphorylation. These results suggest that hyperoxidation of specific Prx isoforms is associated with distinct cell signaling events and identify Prx3 redox status as an important regulator of anabolic and catabolic signal transduction. Targeted strategies to prevent mitochondrial Prx3 hyperoxidation could be useful in maintaining cellular redox balance and homeostatic signaling.

Original languageEnglish (US)
Pages (from-to)139-152
Number of pages14
JournalFree Radical Biology and Medicine
Volume134
DOIs
StatePublished - Apr 2019
Externally publishedYes

Keywords

  • Cartilage
  • MAP kinase signaling
  • Osteoarthritis
  • Oxidative stress
  • Peroxiredoxin
  • Redox signaling

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

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