TY - JOUR
T1 - Differential induction of polyamine oxidase activity in liver and heart of iron-overloaded rats
AU - Tipnis, Ulka R.
AU - He, Cui Ying
AU - Khan, M. Firoze
N1 - Funding Information:
Received 7 June 1996; sent for revision 22 July 1996; accepted 12 September 1996. This investigation was supported by funding awarded to Dr. M. F. Khan (National Institute of Environmental Health Sciences, ES 06476) and Dr. Ulka R. Tipnis (University of Texas Medical Branch Small Grant Program). Address correspondence to Ulka R. Tipnis, PhD, Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA.
PY - 1997/6
Y1 - 1997/6
N2 - The present study was undertaken to investigate the effect of iron dextran treatment on polyamine oxidase (PAO) activity, iron accumulation, and lipid peroxidation in livers and hearts of rats. PAO catalyzes oxidative deamination of polyamines, the cellular aliphatic cations. This reaction produces highly toxic hydrogen peroxide, 3-acetamidopropanal, and precursors of higher polyamines. The rats were given iron dextran daily for 7 d. In iron-dextran-treated rats, a marked increase in the hepatic level of iron was associated with enhanced lipid peroxidation and increased PAO activity. Though iron accumulation and lipid peroxidation in the iron-treated rats increased significantly in the heart, PAO activity remained unchanged. The paraffin sections of livers stained with Perls iron stain showed the presence of iron in macrophages and hepatocytes. The sections of hearts showed iron deposits only in macrophages, while myocytes showed no iron staining. These results show that although iron dextran treatment results in accumulation of iron in both liver and heart, it induces PAO activity only in liver. The significance of increased PAO activity in lipid peroxidation and fibrosis in iron-mediated injury is discussed.
AB - The present study was undertaken to investigate the effect of iron dextran treatment on polyamine oxidase (PAO) activity, iron accumulation, and lipid peroxidation in livers and hearts of rats. PAO catalyzes oxidative deamination of polyamines, the cellular aliphatic cations. This reaction produces highly toxic hydrogen peroxide, 3-acetamidopropanal, and precursors of higher polyamines. The rats were given iron dextran daily for 7 d. In iron-dextran-treated rats, a marked increase in the hepatic level of iron was associated with enhanced lipid peroxidation and increased PAO activity. Though iron accumulation and lipid peroxidation in the iron-treated rats increased significantly in the heart, PAO activity remained unchanged. The paraffin sections of livers stained with Perls iron stain showed the presence of iron in macrophages and hepatocytes. The sections of hearts showed iron deposits only in macrophages, while myocytes showed no iron staining. These results show that although iron dextran treatment results in accumulation of iron in both liver and heart, it induces PAO activity only in liver. The significance of increased PAO activity in lipid peroxidation and fibrosis in iron-mediated injury is discussed.
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U2 - 10.1080/00984109708984024
DO - 10.1080/00984109708984024
M3 - Article
C2 - 9183380
AN - SCOPUS:0030975944
SN - 0098-4108
VL - 51
SP - 235
EP - 244
JO - Journal of Toxicology and Environmental Health
JF - Journal of Toxicology and Environmental Health
IS - 3
ER -