Diagnosis of neonatal enterovirus infection by polymerase chain reaction

Mark J. Abzug; Michael Loeffelholz; Harley A. Rotbart

doi:10.1016/S0022-3476(95)70466-3

Diagnosis of neonatal enterovirus infection by polymerase chain reaction

Mark J. Abzug, Michael Loeffelholz, Harley A. Rotbart

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

A 5-hour colorimetric polymerase chain reaction (PCR) assay was more sensitive than viral culture in identifying viral infection in initial serum (13/16 vs 5/16; p = 0.008) and urine (10/16 vs 5/16; p = 0.2) specimens from 16 enterovirus- infected newborn infants, and remained more sensitive throughout their illnesses. Combined sensitivity of serum and urine PCR was 14 of 16 (88%). Results of all acute-phase PCR assays of serum and urine from four neonates with cultures negative for enterovirus were also negative. PCR assay of serum and urine facilitates rapid, accurate diagnosis of neonatal enterovirus infections. (J PEDIATR 1995;126:447-50).

Original language	English (US)
Pages (from-to)	447-450
Number of pages	4
Journal	The Journal of Pediatrics
Volume	126
Issue number	3
DOIs	https://doi.org/10.1016/S0022-3476(95)70466-3
State	Published - Mar 1995
Externally published	Yes

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health

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10.1016/S0022-3476(95)70466-3

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title = "Diagnosis of neonatal enterovirus infection by polymerase chain reaction",

abstract = "A 5-hour colorimetric polymerase chain reaction (PCR) assay was more sensitive than viral culture in identifying viral infection in initial serum (13/16 vs 5/16; p = 0.008) and urine (10/16 vs 5/16; p = 0.2) specimens from 16 enterovirus- infected newborn infants, and remained more sensitive throughout their illnesses. Combined sensitivity of serum and urine PCR was 14 of 16 (88%). Results of all acute-phase PCR assays of serum and urine from four neonates with cultures negative for enterovirus were also negative. PCR assay of serum and urine facilitates rapid, accurate diagnosis of neonatal enterovirus infections. (J PEDIATR 1995;126:447-50).",

author = "Abzug, {Mark J.} and Michael Loeffelholz and Rotbart, {Harley A.}",

note = "Funding Information: Supported by grant RR-69 from the General Clinical Research Centers Program of the Division of Research Resources, National Institutes of health; the Kempe Research Center, Children's Hospital, Denver, Colo.,; and Roche Molecular Systems. Dr. Loeffelholz is a full-time employee of, and Dr. Rotbart a paid consultant for, Roche Molecular Systems, whose products were used for the work discussed in the article. ",

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doi = "10.1016/S0022-3476(95)70466-3",

language = "English (US)",

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T1 - Diagnosis of neonatal enterovirus infection by polymerase chain reaction

AU - Abzug, Mark J.

AU - Loeffelholz, Michael

AU - Rotbart, Harley A.

N1 - Funding Information: Supported by grant RR-69 from the General Clinical Research Centers Program of the Division of Research Resources, National Institutes of health; the Kempe Research Center, Children's Hospital, Denver, Colo.,; and Roche Molecular Systems. Dr. Loeffelholz is a full-time employee of, and Dr. Rotbart a paid consultant for, Roche Molecular Systems, whose products were used for the work discussed in the article.

PY - 1995/3

Y1 - 1995/3

N2 - A 5-hour colorimetric polymerase chain reaction (PCR) assay was more sensitive than viral culture in identifying viral infection in initial serum (13/16 vs 5/16; p = 0.008) and urine (10/16 vs 5/16; p = 0.2) specimens from 16 enterovirus- infected newborn infants, and remained more sensitive throughout their illnesses. Combined sensitivity of serum and urine PCR was 14 of 16 (88%). Results of all acute-phase PCR assays of serum and urine from four neonates with cultures negative for enterovirus were also negative. PCR assay of serum and urine facilitates rapid, accurate diagnosis of neonatal enterovirus infections. (J PEDIATR 1995;126:447-50).

AB - A 5-hour colorimetric polymerase chain reaction (PCR) assay was more sensitive than viral culture in identifying viral infection in initial serum (13/16 vs 5/16; p = 0.008) and urine (10/16 vs 5/16; p = 0.2) specimens from 16 enterovirus- infected newborn infants, and remained more sensitive throughout their illnesses. Combined sensitivity of serum and urine PCR was 14 of 16 (88%). Results of all acute-phase PCR assays of serum and urine from four neonates with cultures negative for enterovirus were also negative. PCR assay of serum and urine facilitates rapid, accurate diagnosis of neonatal enterovirus infections. (J PEDIATR 1995;126:447-50).

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Diagnosis of neonatal enterovirus infection by polymerase chain reaction

Abstract

ASJC Scopus subject areas

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