Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay

H. A. Rotbart; M. H. Sawyer; S. Fast; C. Lewinski; N. Murphy; E. F. Keyser; J. Spadoro; S. Y. Kao; M. Loeffelholz

doi:10.1128/jcm.32.10.2590-2592.1994

Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay

H. A. Rotbart, M. H. Sawyer, S. Fast, C. Lewinski, N. Murphy, E. F. Keyser, J. Spadoro, S. Y. Kao, M. Loeffelholz

Research output: Contribution to journal › Comment/debate › peer-review

182 Scopus citations

Abstract

A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylase. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.

Original language	English (US)
Pages (from-to)	2590-2592
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	32
Issue number	10
DOIs	https://doi.org/10.1128/jcm.32.10.2590-2592.1994
State	Published - 1994
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/jcm.32.10.2590-2592.1994

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title = "Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay",

abstract = "A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylase. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.",

author = "Rotbart, {H. A.} and Sawyer, {M. H.} and S. Fast and C. Lewinski and N. Murphy and Keyser, {E. F.} and J. Spadoro and Kao, {S. Y.} and M. Loeffelholz",

year = "1994",

doi = "10.1128/jcm.32.10.2590-2592.1994",

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T1 - Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay

AU - Rotbart, H. A.

AU - Sawyer, M. H.

AU - Fast, S.

AU - Lewinski, C.

AU - Murphy, N.

AU - Keyser, E. F.

AU - Spadoro, J.

AU - Kao, S. Y.

AU - Loeffelholz, M.

PY - 1994

Y1 - 1994

N2 - A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylase. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.

AB - A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylase. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.

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DO - 10.1128/jcm.32.10.2590-2592.1994

M3 - Comment/debate

C2 - 7814507

AN - SCOPUS:0028129104

SN - 0095-1137

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SP - 2590

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JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 10

ER -

Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay

Abstract

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