TY - JOUR
T1 - Development of a method to isolate circulating tumor cells using mesenchymal-based capture
AU - Bitting, Rhonda L.
AU - Boominathan, Rengasamy
AU - Rao, Chandra
AU - Kemeny, Gabor
AU - Foulk, Brad
AU - Garcia-Blanco, Mariano A.
AU - Connelly, Mark
AU - Armstrong, Andrew J.
N1 - Funding Information:
The work was supported through a joint collaborative research agreement between Duke University and Veridex/Janssen Research and Development. Additional funding for this work was supported by the Robert B. Goergen Prostate Cancer Foundation Young Investigator Award (Armstrong), the National Cancer Institute (PI Garcia-Blanco, 5R01-CA127727-05), and a Department of Defense Physician Research Training Award (PI Armstrong, W81XWH-10-1-0483 ).
PY - 2013/12/1
Y1 - 2013/12/1
N2 - Epithelial tumor cells can become mesenchymal cells and vice versa via phenotypic transitions, a process known as epithelial plasticity. We postulate that during the process of metastasis, circulating tumor cells (CTCs) lose their epithelial phenotype and acquire a mesenchymal phenotype that may not be sufficiently captured by existing epithelial-based CTC technologies. We report here on the development of a novel CTC capture method, based on the biology of epithelial plasticity, which isolates cells based on OB-cadherin cell surface expression. Using this mesenchymal-based assay, OB-cadherin cellular events are detectable in men with metastatic prostate cancer and are less common in healthy volunteers. This method may complement existing epithelial-based methods and may be particularly useful in patients with bone metastases.
AB - Epithelial tumor cells can become mesenchymal cells and vice versa via phenotypic transitions, a process known as epithelial plasticity. We postulate that during the process of metastasis, circulating tumor cells (CTCs) lose their epithelial phenotype and acquire a mesenchymal phenotype that may not be sufficiently captured by existing epithelial-based CTC technologies. We report here on the development of a novel CTC capture method, based on the biology of epithelial plasticity, which isolates cells based on OB-cadherin cell surface expression. Using this mesenchymal-based assay, OB-cadherin cellular events are detectable in men with metastatic prostate cancer and are less common in healthy volunteers. This method may complement existing epithelial-based methods and may be particularly useful in patients with bone metastases.
KW - Circulating tumor cells
KW - Epithelial plasticity
KW - Epithelial-mesenchymal transition
KW - OB-cadherin
KW - Osteomimicry
KW - Prostate cancer
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U2 - 10.1016/j.ymeth.2013.06.034
DO - 10.1016/j.ymeth.2013.06.034
M3 - Article
C2 - 23845299
AN - SCOPUS:84887606330
SN - 1046-2023
VL - 64
SP - 129
EP - 136
JO - Methods
JF - Methods
IS - 2
ER -