Opredelenie termostabil'nogo i termolabil'nogo énterotoksinov v shtammakh Escherichia coli geneticheskimi, biologicheskimi i immunoserologicheskimi metodami.

T. I. Domaradskaia; I. V. Ezepchuk; V. L. Motin; A. V. Mikul'kis; V. G. Korobko

Opredelenie termostabil'nogo i termolabil'nogo énterotoksinov v shtammakh Escherichia coli geneticheskimi, biologicheskimi i immunoserologicheskimi metodami.

Translated title of the contribution: Determination of thermostable and thermolabile enterotoxins in Escherichia coli strains by genetic, biological, and immunoserological methods

T. I. Domaradskaia, I. V. Ezepchuk, V. L. Motin, A. V. Mikul'kis, V. G. Korobko

Research output: Contribution to journal › Article › peer-review

Abstract

The Escherichia coli strains (75) isolated from patients suffering from diarrhea were screened for ability to produce the temperature-labile or stable toxins (ST or LT) by the different techniques (the hybridization with DNA probes, biological, enzyme immunoassay). The majority of tested strains was shown to harbor the tox-genes controlling the synthesis of ST, LT or both enterotoxins. However, the phenotypic expression of the genes was registered in only some of the strains. The hybridization with the DNA probes is noted to be most perspective in the mass screening of toxigenic strains. The DNA probe used contained the fused estA-eltB genes that makes one able to detect the genes for both enterotoxins.

Translated title of the contribution	Determination of thermostable and thermolabile enterotoxins in Escherichia coli strains by genetic, biological, and immunoserological methods
Original language	Russian
Pages (from-to)	11-13
Number of pages	3
Journal	Molekuliarnaia genetika, mikrobiologiia i virusologiia
Issue number	9-10
State	Published - Sep 1992
Externally published	Yes

ASJC Scopus subject areas

General Medicine

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Opredelenie termostabil'nogo i termolabil'nogo énterotoksinov v shtammakh Escherichia coli geneticheskimi, biologicheskimi i immunoserologicheskimi metodami. / Domaradskaia, T. I.; Ezepchuk, I. V.; Motin, V. L. et al.
In: Molekuliarnaia genetika, mikrobiologiia i virusologiia, No. 9-10, 09.1992, p. 11-13.

Research output: Contribution to journal › Article › peer-review

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abstract = "The Escherichia coli strains (75) isolated from patients suffering from diarrhea were screened for ability to produce the temperature-labile or stable toxins (ST or LT) by the different techniques (the hybridization with DNA probes, biological, enzyme immunoassay). The majority of tested strains was shown to harbor the tox-genes controlling the synthesis of ST, LT or both enterotoxins. However, the phenotypic expression of the genes was registered in only some of the strains. The hybridization with the DNA probes is noted to be most perspective in the mass screening of toxigenic strains. The DNA probe used contained the fused estA-eltB genes that makes one able to detect the genes for both enterotoxins.",

author = "Domaradskaia, {T. I.} and Ezepchuk, {I. V.} and Motin, {V. L.} and Mikul'kis, {A. V.} and Korobko, {V. G.}",

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AU - Ezepchuk, I. V.

AU - Motin, V. L.

AU - Mikul'kis, A. V.

AU - Korobko, V. G.

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Opredelenie termostabil'nogo i termolabil'nogo énterotoksinov v shtammakh Escherichia coli geneticheskimi, biologicheskimi i immunoserologicheskimi metodami.

Abstract

ASJC Scopus subject areas

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