Detection of pathological zinc accumulation in neurons: Methods for autopsy, biopsy, and cultured tissue

Sang Won Suh; Kathy Listiack; Brent Bell; Jefferson Chen; Massoud Motamedi; David Silva; Gorm Danscher; William Whetsell; Richard Thompson; Christopher Frederickson

doi:10.1177/002215549904700715

Detection of pathological zinc accumulation in neurons: Methods for autopsy, biopsy, and cultured tissue

Sang Won Suh, Kathy Listiack, Brent Bell, Jefferson Chen, Massoud Motamedi, David Silva, Gorm Danscher, William Whetsell, Richard Thompson, Christopher Frederickson

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Abstract

It has been repeatedly shown that synaptically released zinc contributes to excitotoxic neuronal injury in ischemia, epilepsy, and mechanical head trauma. Such zinc-induced injury leaves an unmistakable 'footprint' in the injured neurons, allowing an easy and unambiguous postmortem diagnosis. This footprint is the presence of weakly bound, histochemically reactive zinc in the cytoplasm of the perikaryon and proximal dendrites. Such staining appears to be a necessary and sufficient marker for zinc-induced neuronal injury. Here we show how to prepare and stain tissue from biopsy, autopsy, or experimental animal sources for maximal contrast and visibility of zinc- injured neurons.

Original language	English (US)
Pages (from-to)	969-972
Number of pages	4
Journal	Journal of Histochemistry and Cytochemistry
Volume	47
Issue number	7
DOIs	https://doi.org/10.1177/002215549904700715
State	Published - Jul 1999

Keywords

Dendrites
Neuronal injury
Perikarya
Staining
Zinc

ASJC Scopus subject areas

Anatomy
Histology

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10.1177/002215549904700715

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title = "Detection of pathological zinc accumulation in neurons: Methods for autopsy, biopsy, and cultured tissue",

abstract = "It has been repeatedly shown that synaptically released zinc contributes to excitotoxic neuronal injury in ischemia, epilepsy, and mechanical head trauma. Such zinc-induced injury leaves an unmistakable 'footprint' in the injured neurons, allowing an easy and unambiguous postmortem diagnosis. This footprint is the presence of weakly bound, histochemically reactive zinc in the cytoplasm of the perikaryon and proximal dendrites. Such staining appears to be a necessary and sufficient marker for zinc-induced neuronal injury. Here we show how to prepare and stain tissue from biopsy, autopsy, or experimental animal sources for maximal contrast and visibility of zinc- injured neurons.",

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T2 - Methods for autopsy, biopsy, and cultured tissue

AU - Suh, Sang Won

AU - Listiack, Kathy

AU - Bell, Brent

AU - Chen, Jefferson

AU - Motamedi, Massoud

AU - Silva, David

AU - Danscher, Gorm

AU - Whetsell, William

AU - Thompson, Richard

AU - Frederickson, Christopher

PY - 1999/7

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N2 - It has been repeatedly shown that synaptically released zinc contributes to excitotoxic neuronal injury in ischemia, epilepsy, and mechanical head trauma. Such zinc-induced injury leaves an unmistakable 'footprint' in the injured neurons, allowing an easy and unambiguous postmortem diagnosis. This footprint is the presence of weakly bound, histochemically reactive zinc in the cytoplasm of the perikaryon and proximal dendrites. Such staining appears to be a necessary and sufficient marker for zinc-induced neuronal injury. Here we show how to prepare and stain tissue from biopsy, autopsy, or experimental animal sources for maximal contrast and visibility of zinc- injured neurons.

AB - It has been repeatedly shown that synaptically released zinc contributes to excitotoxic neuronal injury in ischemia, epilepsy, and mechanical head trauma. Such zinc-induced injury leaves an unmistakable 'footprint' in the injured neurons, allowing an easy and unambiguous postmortem diagnosis. This footprint is the presence of weakly bound, histochemically reactive zinc in the cytoplasm of the perikaryon and proximal dendrites. Such staining appears to be a necessary and sufficient marker for zinc-induced neuronal injury. Here we show how to prepare and stain tissue from biopsy, autopsy, or experimental animal sources for maximal contrast and visibility of zinc- injured neurons.

KW - Dendrites

KW - Neuronal injury

KW - Perikarya

KW - Staining

KW - Zinc

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Detection of pathological zinc accumulation in neurons: Methods for autopsy, biopsy, and cultured tissue

Abstract

Keywords

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