Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children

Y. Okamoto; K. Kudo; K. Shirotori; M. Nakazawa; E. Ito; K. Togawa; J. A. Patel; P. L. Ogra

doi:10.1177/0003489492101s1003

Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children

Y. Okamoto, K. Kudo, K. Shirotori, M. Nakazawa, E. Ito, K. Togawa, J. A. Patel, P. L. Ogra

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The reverse transcriptase-polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (RSV) sequences in middle ear effusions collected from children with otitis media. Sequences of RSV were detected in 21 of 34 samples tested. These samples were collected during and/or after natural outbreaks of RSV infection in the community. In those patients from whose nasopharynges RSV was isolated, the viral sequences were highly detectable (75%) in the effusions. These observations suggest RSV as an important factor in the pathogenesis of otitis media with effusion.

Original language	English (US)
Pages (from-to)	7-10
Number of pages	4
Journal	Annals of Otology, Rhinology and Laryngology
Volume	101
Issue number	10 II
DOIs	https://doi.org/10.1177/0003489492101s1003
State	Published - 1992
Externally published	Yes

Keywords

otitis media with effusion
polymerase chain reaction
respiratory syncytial virus

ASJC Scopus subject areas

Otorhinolaryngology

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10.1177/0003489492101s1003

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title = "Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children",

abstract = "The reverse transcriptase-polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (RSV) sequences in middle ear effusions collected from children with otitis media. Sequences of RSV were detected in 21 of 34 samples tested. These samples were collected during and/or after natural outbreaks of RSV infection in the community. In those patients from whose nasopharynges RSV was isolated, the viral sequences were highly detectable (75%) in the effusions. These observations suggest RSV as an important factor in the pathogenesis of otitis media with effusion.",

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T1 - Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children

AU - Okamoto, Y.

AU - Kudo, K.

AU - Shirotori, K.

AU - Nakazawa, M.

AU - Ito, E.

AU - Togawa, K.

AU - Patel, J. A.

AU - Ogra, P. L.

PY - 1992

Y1 - 1992

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AB - The reverse transcriptase-polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (RSV) sequences in middle ear effusions collected from children with otitis media. Sequences of RSV were detected in 21 of 34 samples tested. These samples were collected during and/or after natural outbreaks of RSV infection in the community. In those patients from whose nasopharynges RSV was isolated, the viral sequences were highly detectable (75%) in the effusions. These observations suggest RSV as an important factor in the pathogenesis of otitis media with effusion.

KW - otitis media with effusion

KW - polymerase chain reaction

KW - respiratory syncytial virus

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Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children

Abstract

Keywords

ASJC Scopus subject areas

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