TY - JOUR
T1 - Detection of entamoeba histolytica by recombinase polymerase amplification
AU - Nair, Gayatri
AU - Rebolledo, Mauricio
AU - Clinton White, A.
AU - Crannell, Zachary
AU - Rebecca Richards-Kortum, R.
AU - Elizabeth Pinilla, A.
AU - Ramírez, Juan David
AU - Consuelo López, M.
AU - Castellanos-Gonzalez, Alejandro
N1 - Publisher Copyright:
Copyright © 2015 by The American Society of Tropical Medicine and Hygiene.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Traditional microscopy approaches are neither sensitive nor specific for Entamoeba histolytica. Antigen assays are more specific, but many cases are missed unless tested by molecular methods. Although polymerase chain reaction (PCR) is effective, the need for sophisticated, expensive equipment, infrastructure, and trained personnel limits its usefulness, especially in the resource-limited, endemic areas. Here, we report development of a recombinase polymerase amplification (RPA) method to detect E. histolytica specifically. Using visual detection by lateral flow (LF), the test was highly sensitive and specific and could be performed without additional equipment. The availability of this inexpensive, sensitive, and field-applicable diagnostic test could facilitate rapid diagnosis and treatment of amebiasis in endemic regions.
AB - Amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Traditional microscopy approaches are neither sensitive nor specific for Entamoeba histolytica. Antigen assays are more specific, but many cases are missed unless tested by molecular methods. Although polymerase chain reaction (PCR) is effective, the need for sophisticated, expensive equipment, infrastructure, and trained personnel limits its usefulness, especially in the resource-limited, endemic areas. Here, we report development of a recombinase polymerase amplification (RPA) method to detect E. histolytica specifically. Using visual detection by lateral flow (LF), the test was highly sensitive and specific and could be performed without additional equipment. The availability of this inexpensive, sensitive, and field-applicable diagnostic test could facilitate rapid diagnosis and treatment of amebiasis in endemic regions.
UR - http://www.scopus.com/inward/record.url?scp=84941659257&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941659257&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.15-0276
DO - 10.4269/ajtmh.15-0276
M3 - Article
C2 - 26123960
AN - SCOPUS:84941659257
SN - 0002-9637
VL - 93
SP - 591
EP - 595
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 3
ER -