Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis

T. B. Saito; A. A. Alfieri; S. R. Wosiacki; F. J. Negrão; H. S.A. Morais; A. F. Alfieri

doi:10.1016/j.rvsc.2005.03.002

Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis

T. B. Saito, A. A. Alfieri, S. R. Wosiacki, F. J. Negrão, H. S.A. Morais, A. F. Alfieri

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

In a prospective study we evaluated the use of the reverse transcriptase-polymerase chain reaction (RT-PCR) in urine samples to diagnose canine distemper virus in dogs with progressive neurological disease. A fragment of the nucleoprotein gene of canine distemper virus was amplified from the urine of 22 distemper dogs. The body fluids and leukocytes of 12 asymptomatic dogs were RT-PCR negative. RT-PCR of urine samples was more sensitive than serum and leukocytes and at least as sensitive as cerebrospinal fluid to screen for distemper in dogs with neurological signs and extraneural systemic signs.

Original language	English (US)
Pages (from-to)	116-119
Number of pages	4
Journal	Research in Veterinary Science
Volume	80
Issue number	1
DOIs	https://doi.org/10.1016/j.rvsc.2005.03.002
State	Published - Feb 2006
Externally published	Yes

Keywords

Canine distemper
Canine distemper virus
Dogs
RT-PCR
Urine

ASJC Scopus subject areas

General Veterinary

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10.1016/j.rvsc.2005.03.002

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title = "Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis",

abstract = "In a prospective study we evaluated the use of the reverse transcriptase-polymerase chain reaction (RT-PCR) in urine samples to diagnose canine distemper virus in dogs with progressive neurological disease. A fragment of the nucleoprotein gene of canine distemper virus was amplified from the urine of 22 distemper dogs. The body fluids and leukocytes of 12 asymptomatic dogs were RT-PCR negative. RT-PCR of urine samples was more sensitive than serum and leukocytes and at least as sensitive as cerebrospinal fluid to screen for distemper in dogs with neurological signs and extraneural systemic signs.",

keywords = "Canine distemper, Canine distemper virus, Dogs, RT-PCR, Urine",

author = "Saito, {T. B.} and Alfieri, {A. A.} and Wosiacki, {S. R.} and Negr{\~a}o, {F. J.} and Morais, {H. S.A.} and Alfieri, {A. F.}",

note = "Funding Information: Research supported by CAPES, CNPq and Funda{\c c}{\~a}o Arauc{\'a}ria (FAP/PR). A.A. Alfieri is recipient of CNPq fellowship.",

year = "2006",

month = feb,

doi = "10.1016/j.rvsc.2005.03.002",

language = "English (US)",

volume = "80",

pages = "116--119",

journal = "Research in Veterinary Science",

issn = "0034-5288",

publisher = "Elsevier",

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AU - Saito, T. B.

AU - Alfieri, A. A.

AU - Wosiacki, S. R.

AU - Negrão, F. J.

AU - Morais, H. S.A.

AU - Alfieri, A. F.

N1 - Funding Information: Research supported by CAPES, CNPq and Fundação Araucária (FAP/PR). A.A. Alfieri is recipient of CNPq fellowship.

PY - 2006/2

Y1 - 2006/2

N2 - In a prospective study we evaluated the use of the reverse transcriptase-polymerase chain reaction (RT-PCR) in urine samples to diagnose canine distemper virus in dogs with progressive neurological disease. A fragment of the nucleoprotein gene of canine distemper virus was amplified from the urine of 22 distemper dogs. The body fluids and leukocytes of 12 asymptomatic dogs were RT-PCR negative. RT-PCR of urine samples was more sensitive than serum and leukocytes and at least as sensitive as cerebrospinal fluid to screen for distemper in dogs with neurological signs and extraneural systemic signs.

AB - In a prospective study we evaluated the use of the reverse transcriptase-polymerase chain reaction (RT-PCR) in urine samples to diagnose canine distemper virus in dogs with progressive neurological disease. A fragment of the nucleoprotein gene of canine distemper virus was amplified from the urine of 22 distemper dogs. The body fluids and leukocytes of 12 asymptomatic dogs were RT-PCR negative. RT-PCR of urine samples was more sensitive than serum and leukocytes and at least as sensitive as cerebrospinal fluid to screen for distemper in dogs with neurological signs and extraneural systemic signs.

KW - Canine distemper

KW - Canine distemper virus

KW - Dogs

KW - RT-PCR

KW - Urine

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Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis

Abstract

Keywords

ASJC Scopus subject areas

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