Abstract
In a prospective study we evaluated the use of the reverse transcriptase-polymerase chain reaction (RT-PCR) in urine samples to diagnose canine distemper virus in dogs with progressive neurological disease. A fragment of the nucleoprotein gene of canine distemper virus was amplified from the urine of 22 distemper dogs. The body fluids and leukocytes of 12 asymptomatic dogs were RT-PCR negative. RT-PCR of urine samples was more sensitive than serum and leukocytes and at least as sensitive as cerebrospinal fluid to screen for distemper in dogs with neurological signs and extraneural systemic signs.
Original language | English (US) |
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Pages (from-to) | 116-119 |
Number of pages | 4 |
Journal | Research in Veterinary Science |
Volume | 80 |
Issue number | 1 |
DOIs | |
State | Published - Feb 2006 |
Externally published | Yes |
Keywords
- Canine distemper
- Canine distemper virus
- Dogs
- RT-PCR
- Urine
ASJC Scopus subject areas
- General Veterinary