Detection of 8-hydroxy-2'-deoxyguanosine In Deoxyribonucleic Acid By The 32p-postlabeling Method

Lee jane W. Lu; Kohfuku Kohda; Fumiyo Tasaka; James A. Hokanson

doi:10.1248/cpb.39.1880

Detection of 8-hydroxy-2'-deoxyguanosine In Deoxyribonucleic Acid By The ³²p-postlabeling Method

Lee jane W. Lu, Kohfuku Kohda, Fumiyo Tasaka, James A. Hokanson

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Using synthesized 8-hydroxy-2'-deoxyguanosine 3'-monophosphate as a marker, the ³²P-postlabeling method was adapted with minimum modifications for the analysis of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) content in deoxyribonucleic acid (DNA). This method allows the analysis of one 8-OH-dG per 10⁴ DNA nucleotides with only 10 pmoles of nucleotides required. The amounts of 8-OH-dG in DNA detected by the postlabeling method correlated well with the electrochemical detection method but were consistently lower.

Original language	English (US)
Pages (from-to)	1880-1882
Number of pages	3
Journal	Chemical and Pharmaceutical Bulletin
Volume	39
Issue number	7
DOIs	https://doi.org/10.1248/cpb.39.1880
State	Published - 1991
Externally published	Yes

Keywords

32P-postlabeling
8-hydroxydeoxyguanosine
DNA
oxidative damage

ASJC Scopus subject areas

General Chemistry
Drug Discovery

Access to Document

10.1248/cpb.39.1880

Cite this

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title = "Detection of 8-hydroxy-2'-deoxyguanosine In Deoxyribonucleic Acid By The 32p-postlabeling Method",

abstract = "Using synthesized 8-hydroxy-2'-deoxyguanosine 3'-monophosphate as a marker, the 32P-postlabeling method was adapted with minimum modifications for the analysis of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) content in deoxyribonucleic acid (DNA). This method allows the analysis of one 8-OH-dG per 104 DNA nucleotides with only 10 pmoles of nucleotides required. The amounts of 8-OH-dG in DNA detected by the postlabeling method correlated well with the electrochemical detection method but were consistently lower.",

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AU - Kohda, Kohfuku

AU - Tasaka, Fumiyo

AU - Hokanson, James A.

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AB - Using synthesized 8-hydroxy-2'-deoxyguanosine 3'-monophosphate as a marker, the 32P-postlabeling method was adapted with minimum modifications for the analysis of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) content in deoxyribonucleic acid (DNA). This method allows the analysis of one 8-OH-dG per 104 DNA nucleotides with only 10 pmoles of nucleotides required. The amounts of 8-OH-dG in DNA detected by the postlabeling method correlated well with the electrochemical detection method but were consistently lower.

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KW - oxidative damage

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