Dengue 2 virus envelope protein expressed by a recombinant vaccinia virus fails to protect monkeys against Dengue

V. Deubel; R. M. Kinney; J. J. Esposito; C. B. Cropp; A. V. Vorndam; T. P. Monath; D. W. Trent

doi:10.1099/0022-1317-69-8-1921

Dengue 2 virus envelope protein expressed by a recombinant vaccinia virus fails to protect monkeys against Dengue

V. Deubel, R. M. Kinney, J. J. Esposito, C. B. Cropp, A. V. Vorndam, T. P. Monath, D. W. Trent

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

A cDNA copy of the dengue (DEN) 2 virus genome region encoding the virion capsid, membrane and envelope structural proteins has been inserted into vaccinia virus (VV) DNA under the control of its 11K late promoter. The DEN-2 envelope protein was expressed and processed in cells infected with the VV recombinant (VV/D2S). No DEN-2 virus antibody response was detected in mice, hamsters or monkeys vaccinated with VV/D2S. Furthermore, a viraemia was observed in recombinant-vaccinated monkeys after challenge with infectious DEN-2 virus.

Original language	English (US)
Pages (from-to)	1921-1929
Number of pages	9
Journal	Journal of General Virology
Volume	69
Issue number	8
DOIs	https://doi.org/10.1099/0022-1317-69-8-1921
State	Published - 1988
Externally published	Yes

ASJC Scopus subject areas

Virology

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abstract = "A cDNA copy of the dengue (DEN) 2 virus genome region encoding the virion capsid, membrane and envelope structural proteins has been inserted into vaccinia virus (VV) DNA under the control of its 11K late promoter. The DEN-2 envelope protein was expressed and processed in cells infected with the VV recombinant (VV/D2S). No DEN-2 virus antibody response was detected in mice, hamsters or monkeys vaccinated with VV/D2S. Furthermore, a viraemia was observed in recombinant-vaccinated monkeys after challenge with infectious DEN-2 virus.",

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T1 - Dengue 2 virus envelope protein expressed by a recombinant vaccinia virus fails to protect monkeys against Dengue

AU - Deubel, V.

AU - Kinney, R. M.

AU - Esposito, J. J.

AU - Cropp, C. B.

AU - Vorndam, A. V.

AU - Monath, T. P.

AU - Trent, D. W.

PY - 1988

Y1 - 1988

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AB - A cDNA copy of the dengue (DEN) 2 virus genome region encoding the virion capsid, membrane and envelope structural proteins has been inserted into vaccinia virus (VV) DNA under the control of its 11K late promoter. The DEN-2 envelope protein was expressed and processed in cells infected with the VV recombinant (VV/D2S). No DEN-2 virus antibody response was detected in mice, hamsters or monkeys vaccinated with VV/D2S. Furthermore, a viraemia was observed in recombinant-vaccinated monkeys after challenge with infectious DEN-2 virus.

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Dengue 2 virus envelope protein expressed by a recombinant vaccinia virus fails to protect monkeys against Dengue

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