Defective excision of pyrimidine dimers and interstrand DNA crosslinks in rad7 and rad23 mutants of Saccharomyces cerevisiae

Raymond D. Miller; Louise Prakash; Satya Prakash

doi:10.1007/BF00332681

Defective excision of pyrimidine dimers and interstrand DNA crosslinks in rad7 and rad23 mutants of Saccharomyces cerevisiae

Raymond D. Miller, Louise Prakash, Satya Prakash

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Abstract

Excision of pyrimidine dimers and interstrand DNA crosslinks was examined in the deletion mutants rad7-Δ1, rad23-Δ1, and rad7-Δ1 rad23-Δ1. These mutants remove pyrimidine dimers and crosslinks much less efficiently than the RAD⁺ strains; only 30-60% of pyrimidine dimers and 25-40% of crosslinks are removed even after prolonged incubation. The rad7 and rad23 mutations may represent defects in protein factors which increase the efficiency of the nicking enzyme complex or make chromatin more accessible to the nicking activity.

Original language	English (US)
Pages (from-to)	235-239
Number of pages	5
Journal	MGG Molecular & General Genetics
Volume	188
Issue number	2
DOIs	https://doi.org/10.1007/BF00332681
State	Published - Dec 1982
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "Defective excision of pyrimidine dimers and interstrand DNA crosslinks in rad7 and rad23 mutants of Saccharomyces cerevisiae",

abstract = "Excision of pyrimidine dimers and interstrand DNA crosslinks was examined in the deletion mutants rad7-Δ1, rad23-Δ1, and rad7-Δ1 rad23-Δ1. These mutants remove pyrimidine dimers and crosslinks much less efficiently than the RAD+ strains; only 30-60% of pyrimidine dimers and 25-40% of crosslinks are removed even after prolonged incubation. The rad7 and rad23 mutations may represent defects in protein factors which increase the efficiency of the nicking enzyme complex or make chromatin more accessible to the nicking activity.",

author = "Miller, {Raymond D.} and Louise Prakash and Satya Prakash",

year = "1982",

month = dec,

doi = "10.1007/BF00332681",

language = "English (US)",

volume = "188",

pages = "235--239",

journal = "MGG Molecular & General Genetics",

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T1 - Defective excision of pyrimidine dimers and interstrand DNA crosslinks in rad7 and rad23 mutants of Saccharomyces cerevisiae

AU - Miller, Raymond D.

AU - Prakash, Louise

AU - Prakash, Satya

PY - 1982/12

Y1 - 1982/12

N2 - Excision of pyrimidine dimers and interstrand DNA crosslinks was examined in the deletion mutants rad7-Δ1, rad23-Δ1, and rad7-Δ1 rad23-Δ1. These mutants remove pyrimidine dimers and crosslinks much less efficiently than the RAD+ strains; only 30-60% of pyrimidine dimers and 25-40% of crosslinks are removed even after prolonged incubation. The rad7 and rad23 mutations may represent defects in protein factors which increase the efficiency of the nicking enzyme complex or make chromatin more accessible to the nicking activity.

AB - Excision of pyrimidine dimers and interstrand DNA crosslinks was examined in the deletion mutants rad7-Δ1, rad23-Δ1, and rad7-Δ1 rad23-Δ1. These mutants remove pyrimidine dimers and crosslinks much less efficiently than the RAD+ strains; only 30-60% of pyrimidine dimers and 25-40% of crosslinks are removed even after prolonged incubation. The rad7 and rad23 mutations may represent defects in protein factors which increase the efficiency of the nicking enzyme complex or make chromatin more accessible to the nicking activity.

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Defective excision of pyrimidine dimers and interstrand DNA crosslinks in rad7 and rad23 mutants of Saccharomyces cerevisiae

Abstract

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