Decrease of GABA-immunoreactive neurons in the amygdala after electrical kindling in the rat

Patrick M. Callahan; Joseph M. Paris; Cunningham Kathryn A.; Patricia Shinnick-Gallagher

doi:10.1016/0006-8993(91)90361-X

Decrease of GABA-immunoreactive neurons in the amygdala after electrical kindling in the rat

Patrick M. Callahan, Joseph M. Paris, Cunningham Kathryn A., Patricia Shinnick-Gallagher

Pharmacology & Toxicology

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

The present study was designed to investigate the effects of electrical kindling in vivo on GABA immunoreactivity (GABA-IR) of the lateral and basolateral amygdaloid nuclei 2-6 months post-stimulation. Male Sprague-Dawley rats were implanted with bipolar electrodes in the basolateral nucleus and stimulated once per day until 3-5 stage 5 seizures were observed. Coronal sections containing the amygdala were processed for GABA-IR using the contralateral side of the brain. Results indicate that, in comparison to controls. fully kindled animals showed a significant decrease in total number of GABA-IR amygdala neurons. Decreases in GABA-positive punctate structures surrounding unlabeled pyramidal cells were also observed, but not quantified. The present data suggest that epileptogenesis of the amygdala is associated with a significant reduction of GABA-IR in the lateral and basolateral areas throughout the contralateral amygdaloid nucleus.

Original language	English (US)
Pages (from-to)	335-339
Number of pages	5
Journal	Brain Research
Volume	555
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(91)90361-X
State	Published - Aug 2 1991

Keywords

Amygdala
Electrical kindling
Epilepsy
Immunohistochemistry
Rat
γ-Aminobutyric acid

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(91)90361-X

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title = "Decrease of GABA-immunoreactive neurons in the amygdala after electrical kindling in the rat",

abstract = "The present study was designed to investigate the effects of electrical kindling in vivo on GABA immunoreactivity (GABA-IR) of the lateral and basolateral amygdaloid nuclei 2-6 months post-stimulation. Male Sprague-Dawley rats were implanted with bipolar electrodes in the basolateral nucleus and stimulated once per day until 3-5 stage 5 seizures were observed. Coronal sections containing the amygdala were processed for GABA-IR using the contralateral side of the brain. Results indicate that, in comparison to controls. fully kindled animals showed a significant decrease in total number of GABA-IR amygdala neurons. Decreases in GABA-positive punctate structures surrounding unlabeled pyramidal cells were also observed, but not quantified. The present data suggest that epileptogenesis of the amygdala is associated with a significant reduction of GABA-IR in the lateral and basolateral areas throughout the contralateral amygdaloid nucleus.",

keywords = "Amygdala, Electrical kindling, Epilepsy, Immunohistochemistry, Rat, γ-Aminobutyric acid",

author = "Callahan, {Patrick M.} and Paris, {Joseph M.} and {Kathryn A.}, Cunningham and Patricia Shinnick-Gallagher",

note = "Funding Information: The authors gratefully acknowledge Dr. Donald G. Rainnie and Eftihia K. Asprodini for their excellent comments and technical assistance. Support for this research was provided by NIDA Grant DA05708 (K.A.C.) and NIH Grant NS24643 (P.S.G.).",

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doi = "10.1016/0006-8993(91)90361-X",

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AU - Callahan, Patrick M.

AU - Paris, Joseph M.

AU - Kathryn A., Cunningham

AU - Shinnick-Gallagher, Patricia

N1 - Funding Information: The authors gratefully acknowledge Dr. Donald G. Rainnie and Eftihia K. Asprodini for their excellent comments and technical assistance. Support for this research was provided by NIDA Grant DA05708 (K.A.C.) and NIH Grant NS24643 (P.S.G.).

PY - 1991/8/2

Y1 - 1991/8/2

N2 - The present study was designed to investigate the effects of electrical kindling in vivo on GABA immunoreactivity (GABA-IR) of the lateral and basolateral amygdaloid nuclei 2-6 months post-stimulation. Male Sprague-Dawley rats were implanted with bipolar electrodes in the basolateral nucleus and stimulated once per day until 3-5 stage 5 seizures were observed. Coronal sections containing the amygdala were processed for GABA-IR using the contralateral side of the brain. Results indicate that, in comparison to controls. fully kindled animals showed a significant decrease in total number of GABA-IR amygdala neurons. Decreases in GABA-positive punctate structures surrounding unlabeled pyramidal cells were also observed, but not quantified. The present data suggest that epileptogenesis of the amygdala is associated with a significant reduction of GABA-IR in the lateral and basolateral areas throughout the contralateral amygdaloid nucleus.

AB - The present study was designed to investigate the effects of electrical kindling in vivo on GABA immunoreactivity (GABA-IR) of the lateral and basolateral amygdaloid nuclei 2-6 months post-stimulation. Male Sprague-Dawley rats were implanted with bipolar electrodes in the basolateral nucleus and stimulated once per day until 3-5 stage 5 seizures were observed. Coronal sections containing the amygdala were processed for GABA-IR using the contralateral side of the brain. Results indicate that, in comparison to controls. fully kindled animals showed a significant decrease in total number of GABA-IR amygdala neurons. Decreases in GABA-positive punctate structures surrounding unlabeled pyramidal cells were also observed, but not quantified. The present data suggest that epileptogenesis of the amygdala is associated with a significant reduction of GABA-IR in the lateral and basolateral areas throughout the contralateral amygdaloid nucleus.

KW - Amygdala

KW - Electrical kindling

KW - Epilepsy

KW - Immunohistochemistry

KW - Rat

KW - γ-Aminobutyric acid

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Decrease of GABA-immunoreactive neurons in the amygdala after electrical kindling in the rat

Abstract

Keywords

ASJC Scopus subject areas

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