Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment

Michael B. Sherman; Juan Trujillo; Benjamin E. Bammes; Liang Jin; Matthias W. Stumpf; Scott C. Weaver

doi:10.3934/biophy.2015.2.153

Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment

Michael B. Sherman, Juan Trujillo, Benjamin E. Bammes, Liang Jin, Matthias W. Stumpf, Scott C. Weaver

Research output: Contribution to journal › Article › peer-review

Abstract

A unique biological safety level (BSL)-3 cryo-electron microscopy facility with a 200 keV high-end cryo-electron microscope has been commissioned at the University of Texas Medical Branch (UTMB) to study the structure of viruses and bacteria classified as select agents. We developed a microscope decontamination protocol based on chlorine dioxide gas with a continuous flow system. In this paper we report on testing digital camera sensors (both CCD and CMOS direct detector) in a BSL-3 environment, and microscope performance after chlorine dioxide (ClO2) decontamination cycles.

Original language	English (US)
Pages (from-to)	153-162
Number of pages	10
Journal	AIMS Biophysics
Volume	2
Issue number	2
DOIs	https://doi.org/10.3934/biophy.2015.2.153
State	Published - 2015

Keywords

Biological safety containment
Cryo-electron microscopy
Single particle imaging

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology

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10.3934/biophy.2015.2.153

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abstract = "A unique biological safety level (BSL)-3 cryo-electron microscopy facility with a 200 keV high-end cryo-electron microscope has been commissioned at the University of Texas Medical Branch (UTMB) to study the structure of viruses and bacteria classified as select agents. We developed a microscope decontamination protocol based on chlorine dioxide gas with a continuous flow system. In this paper we report on testing digital camera sensors (both CCD and CMOS direct detector) in a BSL-3 environment, and microscope performance after chlorine dioxide (ClO2) decontamination cycles.",

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AU - Jin, Liang

AU - Stumpf, Matthias W.

AU - Weaver, Scott C.

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AB - A unique biological safety level (BSL)-3 cryo-electron microscopy facility with a 200 keV high-end cryo-electron microscope has been commissioned at the University of Texas Medical Branch (UTMB) to study the structure of viruses and bacteria classified as select agents. We developed a microscope decontamination protocol based on chlorine dioxide gas with a continuous flow system. In this paper we report on testing digital camera sensors (both CCD and CMOS direct detector) in a BSL-3 environment, and microscope performance after chlorine dioxide (ClO2) decontamination cycles.

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KW - Cryo-electron microscopy

KW - Single particle imaging

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Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment

Abstract

Keywords

ASJC Scopus subject areas

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