Cytogenetic effects of cis-platinum(II)diamminedichloride on human lymphocyte cultures

Julianne Meyne; Lillian H. Lockhart

doi:10.1016/0165-1218(78)90098-8

Cytogenetic effects of cis-platinum(II)diamminedichloride on human lymphocyte cultures

Julianne Meyne, Lillian H. Lockhart

Pediatrics

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Human lymphocytes were treated with the antitumor agent cis-platinum(II)-diamminedichloride (PDD) during either the last 24 h or 48 h of incubation. A dose-dependent effect was observed for both inhibition of mitotic activity and increased frequency of metaphases with chromosomal aberrations. The aberrations observed consisted primarily of chromatid breaks. Statistical analysis of 3244 PDD-induced breakpoints demonstrated a significantly non-random distribution of breakpoints between chromosomes. The pattern of distribution varied with the type of aberration. Only chromosome number 9 had a significant increase of breakpoints for each type of aberration analyzed. The breakpoints were located predominantly in lightly staining G-bands. Certain individual bands had relatively high frequencies of breakpoints, indicating a specific interaction occurs between PDD and the DNA of human lymphocytes in vitro.

Original language	English (US)
Pages (from-to)	87-97
Number of pages	11
Journal	Mutation Research/Genetic Toxicology
Volume	58
Issue number	1
DOIs	https://doi.org/10.1016/0165-1218(78)90098-8
State	Published - Sep 1978

ASJC Scopus subject areas

Toxicology
Genetics

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@article{467a6d3016d8459f9d74c137cd49651a,

title = "Cytogenetic effects of cis-platinum(II)diamminedichloride on human lymphocyte cultures",

abstract = "Human lymphocytes were treated with the antitumor agent cis-platinum(II)-diamminedichloride (PDD) during either the last 24 h or 48 h of incubation. A dose-dependent effect was observed for both inhibition of mitotic activity and increased frequency of metaphases with chromosomal aberrations. The aberrations observed consisted primarily of chromatid breaks. Statistical analysis of 3244 PDD-induced breakpoints demonstrated a significantly non-random distribution of breakpoints between chromosomes. The pattern of distribution varied with the type of aberration. Only chromosome number 9 had a significant increase of breakpoints for each type of aberration analyzed. The breakpoints were located predominantly in lightly staining G-bands. Certain individual bands had relatively high frequencies of breakpoints, indicating a specific interaction occurs between PDD and the DNA of human lymphocytes in vitro.",

author = "Julianne Meyne and Lockhart, {Lillian H.}",

note = "Funding Information: This research was supported in part by Special Project 409, Children's Bureau, DHEW (now Project 240-Interdisciplinary Care: The Handicapped Child in His Family) and by the National Foundation Birth Defects Grant. During the time the research was being done J.M. held an American Association of University Women Predoctoral Fellowship (Edna Rowe Endowed).",

year = "1978",

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doi = "10.1016/0165-1218(78)90098-8",

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AU - Lockhart, Lillian H.

N1 - Funding Information: This research was supported in part by Special Project 409, Children's Bureau, DHEW (now Project 240-Interdisciplinary Care: The Handicapped Child in His Family) and by the National Foundation Birth Defects Grant. During the time the research was being done J.M. held an American Association of University Women Predoctoral Fellowship (Edna Rowe Endowed).

PY - 1978/9

Y1 - 1978/9

N2 - Human lymphocytes were treated with the antitumor agent cis-platinum(II)-diamminedichloride (PDD) during either the last 24 h or 48 h of incubation. A dose-dependent effect was observed for both inhibition of mitotic activity and increased frequency of metaphases with chromosomal aberrations. The aberrations observed consisted primarily of chromatid breaks. Statistical analysis of 3244 PDD-induced breakpoints demonstrated a significantly non-random distribution of breakpoints between chromosomes. The pattern of distribution varied with the type of aberration. Only chromosome number 9 had a significant increase of breakpoints for each type of aberration analyzed. The breakpoints were located predominantly in lightly staining G-bands. Certain individual bands had relatively high frequencies of breakpoints, indicating a specific interaction occurs between PDD and the DNA of human lymphocytes in vitro.

AB - Human lymphocytes were treated with the antitumor agent cis-platinum(II)-diamminedichloride (PDD) during either the last 24 h or 48 h of incubation. A dose-dependent effect was observed for both inhibition of mitotic activity and increased frequency of metaphases with chromosomal aberrations. The aberrations observed consisted primarily of chromatid breaks. Statistical analysis of 3244 PDD-induced breakpoints demonstrated a significantly non-random distribution of breakpoints between chromosomes. The pattern of distribution varied with the type of aberration. Only chromosome number 9 had a significant increase of breakpoints for each type of aberration analyzed. The breakpoints were located predominantly in lightly staining G-bands. Certain individual bands had relatively high frequencies of breakpoints, indicating a specific interaction occurs between PDD and the DNA of human lymphocytes in vitro.

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Cytogenetic effects of cis-platinum(II)diamminedichloride on human lymphocyte cultures

Abstract

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