Crystallization and preliminary X-ray diffraction of the ZO-binding domain of human occludin

Bi Hung Peng; Mark A. White; Gerald A. Campbell; Jebamony J. Robert; J. Ching Lee; Roger B. Sutton

doi:10.1107/S1744309105007475

Crystallization and preliminary X-ray diffraction of the ZO-binding domain of human occludin

Bi Hung Peng, Mark A. White, Gerald A. Campbell, Jebamony J. Robert, J. Ching Lee, Roger B. Sutton

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Occludin is a tight-junction protein controlling the integrity of endothelial and epithelial cell layers. It forms complexes with the cytoplasmic proteins ZO-1, ZO-2 and ZO-3. The ZO-binding domain in the C-terminal cytoplasmic region of human occludin has previously been isolated and identified. This domain, as expressed in a bacterial system or isolated from native cellular occludin, maintains its ability to bind ZO-1 and ZO-2. The crystallization conditions of the human ZO-binding domain are reported here. The crystals diffract to 2.3 Å resolution and were shown to belong to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 33.3, b = 35.4, c = 107.3 Å.

Original language	English (US)
Pages (from-to)	369-371
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	61
Issue number	4
DOIs	https://doi.org/10.1107/S1744309105007475
State	Published - 2005

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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title = "Crystallization and preliminary X-ray diffraction of the ZO-binding domain of human occludin",

abstract = "Occludin is a tight-junction protein controlling the integrity of endothelial and epithelial cell layers. It forms complexes with the cytoplasmic proteins ZO-1, ZO-2 and ZO-3. The ZO-binding domain in the C-terminal cytoplasmic region of human occludin has previously been isolated and identified. This domain, as expressed in a bacterial system or isolated from native cellular occludin, maintains its ability to bind ZO-1 and ZO-2. The crystallization conditions of the human ZO-binding domain are reported here. The crystals diffract to 2.3 {\AA} resolution and were shown to belong to the orthorhombic space group P212121, with unit-cell parameters a = 33.3, b = 35.4, c = 107.3 {\AA}.",

author = "Peng, {Bi Hung} and White, {Mark A.} and Campbell, {Gerald A.} and Robert, {Jebamony J.} and Lee, {J. Ching} and Sutton, {Roger B.}",

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TY - JOUR

T1 - Crystallization and preliminary X-ray diffraction of the ZO-binding domain of human occludin

AU - Peng, Bi Hung

AU - White, Mark A.

AU - Campbell, Gerald A.

AU - Robert, Jebamony J.

AU - Lee, J. Ching

AU - Sutton, Roger B.

PY - 2005

Y1 - 2005

N2 - Occludin is a tight-junction protein controlling the integrity of endothelial and epithelial cell layers. It forms complexes with the cytoplasmic proteins ZO-1, ZO-2 and ZO-3. The ZO-binding domain in the C-terminal cytoplasmic region of human occludin has previously been isolated and identified. This domain, as expressed in a bacterial system or isolated from native cellular occludin, maintains its ability to bind ZO-1 and ZO-2. The crystallization conditions of the human ZO-binding domain are reported here. The crystals diffract to 2.3 Å resolution and were shown to belong to the orthorhombic space group P212121, with unit-cell parameters a = 33.3, b = 35.4, c = 107.3 Å.

AB - Occludin is a tight-junction protein controlling the integrity of endothelial and epithelial cell layers. It forms complexes with the cytoplasmic proteins ZO-1, ZO-2 and ZO-3. The ZO-binding domain in the C-terminal cytoplasmic region of human occludin has previously been isolated and identified. This domain, as expressed in a bacterial system or isolated from native cellular occludin, maintains its ability to bind ZO-1 and ZO-2. The crystallization conditions of the human ZO-binding domain are reported here. The crystals diffract to 2.3 Å resolution and were shown to belong to the orthorhombic space group P212121, with unit-cell parameters a = 33.3, b = 35.4, c = 107.3 Å.

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Crystallization and preliminary X-ray diffraction of the ZO-binding domain of human occludin

Abstract

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