Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG): An essential mediator of merozoite egress

Samantha Nava; Aygul Sadiqova; Alejandro Castellanos-Gonzalez; A. Clinton White

doi:10.1016/j.molbiopara.2020.111277

Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG): An essential mediator of merozoite egress

Samantha Nava, Aygul Sadiqova, Alejandro Castellanos-Gonzalez, A. Clinton White

Internal Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Cryptosporidiosis is an obligate intracellular pathogen causing diarrhea. Merozoite egress is essential for infection to spread between host cells. However, the mechanisms of egress have yet to be defined. We hypothesized that Cyclic GMP-Dependent Protein Kinase G (PKG) may be involved in Cryptosporidium egress. In this study, Cryptosporidium parvum PKG was silenced by using antisense RNA sequences. PKG-silencing significantly inhibited egress of merozoites from infected HCT-8 cells into the supernatant and led to retention of intracellular forms within the host cells. This data identifies PKG as a key mediator of merozoite egress, a key step in the parasite lifecycle.

Original language	English (US)
Article number	111277
Journal	Molecular and Biochemical Parasitology
Volume	237
DOIs	https://doi.org/10.1016/j.molbiopara.2020.111277
State	Published - May 2020

Keywords

Cryptosporidium
Cyclic GMP-dependent protein kinase
Egress

ASJC Scopus subject areas

Parasitology
Molecular Biology

Access to Document

10.1016/j.molbiopara.2020.111277

Cite this

@article{02d2b9359a294b399fb3f7c851379234,

title = "Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG): An essential mediator of merozoite egress",

abstract = "Cryptosporidiosis is an obligate intracellular pathogen causing diarrhea. Merozoite egress is essential for infection to spread between host cells. However, the mechanisms of egress have yet to be defined. We hypothesized that Cyclic GMP-Dependent Protein Kinase G (PKG) may be involved in Cryptosporidium egress. In this study, Cryptosporidium parvum PKG was silenced by using antisense RNA sequences. PKG-silencing significantly inhibited egress of merozoites from infected HCT-8 cells into the supernatant and led to retention of intracellular forms within the host cells. This data identifies PKG as a key mediator of merozoite egress, a key step in the parasite lifecycle.",

keywords = "Cryptosporidium, Cyclic GMP-dependent protein kinase, Egress",

author = "Samantha Nava and Aygul Sadiqova and Alejandro Castellanos-Gonzalez and White, {A. Clinton}",

note = "Publisher Copyright: {\textcopyright} 2020 The Author(s)",

year = "2020",

month = may,

doi = "10.1016/j.molbiopara.2020.111277",

language = "English (US)",

volume = "237",

journal = "Molecular and Biochemical Parasitology",

issn = "0166-6851",

publisher = "Elsevier",

}

TY - JOUR

T1 - Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG)

T2 - An essential mediator of merozoite egress

AU - Nava, Samantha

AU - Sadiqova, Aygul

AU - Castellanos-Gonzalez, Alejandro

AU - White, A. Clinton

PY - 2020/5

Y1 - 2020/5

N2 - Cryptosporidiosis is an obligate intracellular pathogen causing diarrhea. Merozoite egress is essential for infection to spread between host cells. However, the mechanisms of egress have yet to be defined. We hypothesized that Cyclic GMP-Dependent Protein Kinase G (PKG) may be involved in Cryptosporidium egress. In this study, Cryptosporidium parvum PKG was silenced by using antisense RNA sequences. PKG-silencing significantly inhibited egress of merozoites from infected HCT-8 cells into the supernatant and led to retention of intracellular forms within the host cells. This data identifies PKG as a key mediator of merozoite egress, a key step in the parasite lifecycle.

AB - Cryptosporidiosis is an obligate intracellular pathogen causing diarrhea. Merozoite egress is essential for infection to spread between host cells. However, the mechanisms of egress have yet to be defined. We hypothesized that Cyclic GMP-Dependent Protein Kinase G (PKG) may be involved in Cryptosporidium egress. In this study, Cryptosporidium parvum PKG was silenced by using antisense RNA sequences. PKG-silencing significantly inhibited egress of merozoites from infected HCT-8 cells into the supernatant and led to retention of intracellular forms within the host cells. This data identifies PKG as a key mediator of merozoite egress, a key step in the parasite lifecycle.

KW - Cryptosporidium

KW - Cyclic GMP-dependent protein kinase

KW - Egress

UR - http://www.scopus.com/inward/record.url?scp=85084346223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85084346223&partnerID=8YFLogxK

U2 - 10.1016/j.molbiopara.2020.111277

DO - 10.1016/j.molbiopara.2020.111277

M3 - Article

C2 - 32348840

AN - SCOPUS:85084346223

SN - 0166-6851

VL - 237

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

M1 - 111277

ER -

Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG): An essential mediator of merozoite egress

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this