Cross-protection between distantly related spotted fever group rickettsiae

Hui Min Feng; David H. Walker

doi:10.1016/S0264-410X(03)00301-3

Cross-protection between distantly related spotted fever group rickettsiae

Hui Min Feng, David H. Walker

Pathology

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Two excellent C3H/HeN mouse models of spotted fever rickettsioses caused by the distantly related organisms, Rickettsia conorii and Rickettsia australis, were utilized to evaluate the possibility of the stimulation of broad cross-protective immunity. Sublethal infection stimulated complete immunity, that is absence of disease, after challenge with a dose of the heterologous Rickettsia that uniformly killed naïve mice. In contrast, heterologous immune sera did not protect mice against a lethal dose (two LD₅₀) of rickettsiae in the mouse toxicity neutralization assay, the standard method for evaluation of rickettsial vaccine potency. These observations suggest that development of a broadly protective vaccine against spotted fever group (SFG) rickettsiae is feasible, and the results indicate that mouse toxicity neutralization is an inappropriate method for evaluation of candidate vaccines.

Original language	English (US)
Pages (from-to)	3901-3905
Number of pages	5
Journal	Vaccine
Volume	21
Issue number	25-26
DOIs	https://doi.org/10.1016/S0264-410X(03)00301-3
State	Published - 2003

Keywords

Mouse models
Protective immunity
Rickettsia australis
Rickettsia conorii
Toxicity neutralization

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/S0264-410X(03)00301-3

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title = "Cross-protection between distantly related spotted fever group rickettsiae",

abstract = "Two excellent C3H/HeN mouse models of spotted fever rickettsioses caused by the distantly related organisms, Rickettsia conorii and Rickettsia australis, were utilized to evaluate the possibility of the stimulation of broad cross-protective immunity. Sublethal infection stimulated complete immunity, that is absence of disease, after challenge with a dose of the heterologous Rickettsia that uniformly killed na{\"i}ve mice. In contrast, heterologous immune sera did not protect mice against a lethal dose (two LD50) of rickettsiae in the mouse toxicity neutralization assay, the standard method for evaluation of rickettsial vaccine potency. These observations suggest that development of a broadly protective vaccine against spotted fever group (SFG) rickettsiae is feasible, and the results indicate that mouse toxicity neutralization is an inappropriate method for evaluation of candidate vaccines.",

keywords = "Mouse models, Protective immunity, Rickettsia australis, Rickettsia conorii, Toxicity neutralization",

author = "Feng, {Hui Min} and Walker, {David H.}",

note = "Funding Information: The authors wish to thank Kay Kantowski for expert assistance in the preparation of the manuscript and Gui-Min He for statistical assistance. This research was funded by a grant from the National Institute of Allergy and Infectious Diseases Health (AI 21242).",

year = "2003",

doi = "10.1016/S0264-410X(03)00301-3",

language = "English (US)",

volume = "21",

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journal = "Vaccine",

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T1 - Cross-protection between distantly related spotted fever group rickettsiae

AU - Feng, Hui Min

AU - Walker, David H.

N1 - Funding Information: The authors wish to thank Kay Kantowski for expert assistance in the preparation of the manuscript and Gui-Min He for statistical assistance. This research was funded by a grant from the National Institute of Allergy and Infectious Diseases Health (AI 21242).

PY - 2003

Y1 - 2003

N2 - Two excellent C3H/HeN mouse models of spotted fever rickettsioses caused by the distantly related organisms, Rickettsia conorii and Rickettsia australis, were utilized to evaluate the possibility of the stimulation of broad cross-protective immunity. Sublethal infection stimulated complete immunity, that is absence of disease, after challenge with a dose of the heterologous Rickettsia that uniformly killed naïve mice. In contrast, heterologous immune sera did not protect mice against a lethal dose (two LD50) of rickettsiae in the mouse toxicity neutralization assay, the standard method for evaluation of rickettsial vaccine potency. These observations suggest that development of a broadly protective vaccine against spotted fever group (SFG) rickettsiae is feasible, and the results indicate that mouse toxicity neutralization is an inappropriate method for evaluation of candidate vaccines.

AB - Two excellent C3H/HeN mouse models of spotted fever rickettsioses caused by the distantly related organisms, Rickettsia conorii and Rickettsia australis, were utilized to evaluate the possibility of the stimulation of broad cross-protective immunity. Sublethal infection stimulated complete immunity, that is absence of disease, after challenge with a dose of the heterologous Rickettsia that uniformly killed naïve mice. In contrast, heterologous immune sera did not protect mice against a lethal dose (two LD50) of rickettsiae in the mouse toxicity neutralization assay, the standard method for evaluation of rickettsial vaccine potency. These observations suggest that development of a broadly protective vaccine against spotted fever group (SFG) rickettsiae is feasible, and the results indicate that mouse toxicity neutralization is an inappropriate method for evaluation of candidate vaccines.

KW - Mouse models

KW - Protective immunity

KW - Rickettsia australis

KW - Rickettsia conorii

KW - Toxicity neutralization

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U2 - 10.1016/S0264-410X(03)00301-3

DO - 10.1016/S0264-410X(03)00301-3

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AN - SCOPUS:0242349120

SN - 0264-410X

VL - 21

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EP - 3905

JO - Vaccine

JF - Vaccine

IS - 25-26

ER -

Cross-protection between distantly related spotted fever group rickettsiae

Abstract

Keywords

ASJC Scopus subject areas

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