Corrigendum to: Nup42 and IP 6 coordinate Gle1 stimulation of Dbp5/DDX19B for mRNA export in yeast and human cells (Traffic, (2017), 18, 12, (776-790), 10.1111/tra.12526)

The authors wish to replace Figure in their paper with the figure below. Figure panel C is incorrectly labeled in the original and is corrected here. (Figure presented.) hGle1B interactions with hNup42 and IP₆ enhances stimulation of DDX19B activity and are required for mRNA export. (A) Purified recombinant human proteins used in ATPase assays. 1 μg indicated purified proteins were resolved by SDS-PAGE and Coomassie stained. (B) hGle1B and hNup42 stimulate DDX19B ATPase activity in absence of IP₆. PK/LDH-coupled ATPase assays were performed with H₆-DDX19B (500 nM, expressed in insect cells) in the presence of 1 μM A₃₀ RNA and 2 mM ATP. hgle1B-CTD or hgle1B-CTD^{QKED > AAAA} (250 nM), IP₆ (1 μM), and varying amounts of hnup42-CTD (250, 500, or 750 nM) were added as indicated. Reactions preformed as in Figure 3D. Mean shown for n = 3, and SE is indicated by error bars. (C) hGle1B-IP₆ interaction is conserved in human proteins. PK/LDH-coupled ATPase assays were performed with H₆-DDX19B (500 nM) in the presence of 1 μM A₃₀ RNA and 2 mM ATP, hgle1B-CTD, hgle1B-CTD^{KK > QQ}, or hgle1B-CTD^{KKKK > QQQQ} (250 nM), and hnup42-CTD (750 nM) or IP₆ (1 μM) were added as indicated. Purified proteins are shown in 6A. Reactions performed as in Figure 3D. Mean shown for n = 3, and SE is indicated by error bars. (D) hGle1B-IP₆ interaction is required for mRNA export. HeLa cells were transfected with vehicle or GFP-hGLE1B^R, GFP-hgle1B^{R-KK > QQ}, or GFP-hgle1B^{R-KKKK > QQQQ} plasmids following CTRL or hGLE1 knockdown. Nuclear:cytoplasmic mean fluorescence intensity of oligo d(T) signal was quantified as in Figure 5F.