Construction of yellow fever/St. Louis encephalitis chimeric virus and the use of chimeras as a diagnostic tool

Konstantin V. Pugachev; Farshad Guirakhoo; Fred Mitchell; Simeon W. Ocran; Megan Parsons; Barbara W. Johnson; Olga L. Kosoy; Robert S. Lanciotti; John T. Roehrig; Dennis W. Trent; Thomas P. Monath

doi:10.4269/ajtmh.2004.71.639

Construction of yellow fever/St. Louis encephalitis chimeric virus and the use of chimeras as a diagnostic tool

Konstantin V. Pugachev, Farshad Guirakhoo, Fred Mitchell, Simeon W. Ocran, Megan Parsons, Barbara W. Johnson, Olga L. Kosoy, Robert S. Lanciotti, John T. Roehrig, Dennis W. Trent, Thomas P. Monath

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

St. Louis encephalitis (SLE) and West Nile (WN) flaviviruses are genetically closely related and co-circulate in the United States. Virus neutralization tests provide the most specific means for serodiagnosis of infections with these viruses. However, use of wild-type SLE and WN viral strains for laboratory testing is constrained by the biocontainment requirements. We constructed two highly attenuated yellow fever (YF) virus chimeras that contain the premembrane-envelope (prM-E) protein genes from the virulent MSI-7 (isolated in the United States) or the naturally attenuated CorAn9124 (Argentina) SLE strains. The YF/SLE (CorAn version) virus and the previously constructed YF/WN chimera were shown to specifically distinguish between confirmed human SLE and WN cases in a virus neutralization test using patient sera. These chimeras have the potential for use as diagnostic reagents and vaccines against SLE and WN.

Original language	English (US)
Pages (from-to)	639-645
Number of pages	7
Journal	American Journal of Tropical Medicine and Hygiene
Volume	71
Issue number	5
DOIs	https://doi.org/10.4269/ajtmh.2004.71.639
State	Published - Nov 2004
Externally published	Yes

ASJC Scopus subject areas

Parasitology
Virology
Infectious Diseases

Access to Document

10.4269/ajtmh.2004.71.639

Cite this

Pugachev, K. V., Guirakhoo, F., Mitchell, F., Ocran, S. W., Parsons, M., Johnson, B. W., Kosoy, O. L., Lanciotti, R. S., Roehrig, J. T., Trent, D. W., & Monath, T. P. (2004). Construction of yellow fever/St. Louis encephalitis chimeric virus and the use of chimeras as a diagnostic tool. American Journal of Tropical Medicine and Hygiene, 71(5), 639-645. https://doi.org/10.4269/ajtmh.2004.71.639

Pugachev, KV, Guirakhoo, F, Mitchell, F, Ocran, SW, Parsons, M, Johnson, BW, Kosoy, OL, Lanciotti, RS, Roehrig, JT, Trent, DW & Monath, TP 2004, 'Construction of yellow fever/St. Louis encephalitis chimeric virus and the use of chimeras as a diagnostic tool', American Journal of Tropical Medicine and Hygiene, vol. 71, no. 5, pp. 639-645. https://doi.org/10.4269/ajtmh.2004.71.639

@article{e22d276e6cd846dc9ddac374e967e960,

title = "Construction of yellow fever/St. Louis encephalitis chimeric virus and the use of chimeras as a diagnostic tool",

abstract = "St. Louis encephalitis (SLE) and West Nile (WN) flaviviruses are genetically closely related and co-circulate in the United States. Virus neutralization tests provide the most specific means for serodiagnosis of infections with these viruses. However, use of wild-type SLE and WN viral strains for laboratory testing is constrained by the biocontainment requirements. We constructed two highly attenuated yellow fever (YF) virus chimeras that contain the premembrane-envelope (prM-E) protein genes from the virulent MSI-7 (isolated in the United States) or the naturally attenuated CorAn9124 (Argentina) SLE strains. The YF/SLE (CorAn version) virus and the previously constructed YF/WN chimera were shown to specifically distinguish between confirmed human SLE and WN cases in a virus neutralization test using patient sera. These chimeras have the potential for use as diagnostic reagents and vaccines against SLE and WN.",

author = "Pugachev, {Konstantin V.} and Farshad Guirakhoo and Fred Mitchell and Ocran, {Simeon W.} and Megan Parsons and Johnson, {Barbara W.} and Kosoy, {Olga L.} and Lanciotti, {Robert S.} and Roehrig, {John T.} and Trent, {Dennis W.} and Monath, {Thomas P.}",

year = "2004",

month = nov,

doi = "10.4269/ajtmh.2004.71.639",

language = "English (US)",

volume = "71",

pages = "639--645",

journal = "American Journal of Tropical Medicine and Hygiene",

issn = "0002-9637",

publisher = "American Society of Tropical Medicine and Hygiene",

number = "5",

}

TY - JOUR

T1 - Construction of yellow fever/St. Louis encephalitis chimeric virus and the use of chimeras as a diagnostic tool

AU - Pugachev, Konstantin V.

AU - Guirakhoo, Farshad

AU - Mitchell, Fred

AU - Ocran, Simeon W.

AU - Parsons, Megan

AU - Johnson, Barbara W.

AU - Kosoy, Olga L.

AU - Lanciotti, Robert S.

AU - Roehrig, John T.

AU - Trent, Dennis W.

AU - Monath, Thomas P.

PY - 2004/11

Y1 - 2004/11

N2 - St. Louis encephalitis (SLE) and West Nile (WN) flaviviruses are genetically closely related and co-circulate in the United States. Virus neutralization tests provide the most specific means for serodiagnosis of infections with these viruses. However, use of wild-type SLE and WN viral strains for laboratory testing is constrained by the biocontainment requirements. We constructed two highly attenuated yellow fever (YF) virus chimeras that contain the premembrane-envelope (prM-E) protein genes from the virulent MSI-7 (isolated in the United States) or the naturally attenuated CorAn9124 (Argentina) SLE strains. The YF/SLE (CorAn version) virus and the previously constructed YF/WN chimera were shown to specifically distinguish between confirmed human SLE and WN cases in a virus neutralization test using patient sera. These chimeras have the potential for use as diagnostic reagents and vaccines against SLE and WN.

AB - St. Louis encephalitis (SLE) and West Nile (WN) flaviviruses are genetically closely related and co-circulate in the United States. Virus neutralization tests provide the most specific means for serodiagnosis of infections with these viruses. However, use of wild-type SLE and WN viral strains for laboratory testing is constrained by the biocontainment requirements. We constructed two highly attenuated yellow fever (YF) virus chimeras that contain the premembrane-envelope (prM-E) protein genes from the virulent MSI-7 (isolated in the United States) or the naturally attenuated CorAn9124 (Argentina) SLE strains. The YF/SLE (CorAn version) virus and the previously constructed YF/WN chimera were shown to specifically distinguish between confirmed human SLE and WN cases in a virus neutralization test using patient sera. These chimeras have the potential for use as diagnostic reagents and vaccines against SLE and WN.

UR - http://www.scopus.com/inward/record.url?scp=10044276985&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10044276985&partnerID=8YFLogxK

U2 - 10.4269/ajtmh.2004.71.639

DO - 10.4269/ajtmh.2004.71.639

M3 - Article

C2 - 15569798

AN - SCOPUS:10044276985

SN - 0002-9637

VL - 71

SP - 639

EP - 645

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

IS - 5

ER -

Construction of yellow fever/St. Louis encephalitis chimeric virus and the use of chimeras as a diagnostic tool

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this