Conservation of wild animals by assisted reproduction and molecular marker technology

S. Shivaji, S. D. Kholkute, S. K. Verma, Ajay Gaur, G. Umapathy, Anju Singh, Sadanand Sontakke, K. Shailaja, Anuradha Reddy, S. Monika, V. Sivaram, B. Jyotsna, Satyare Bala, M. Shakeel Ahmed, Aruna Bala, B. V.N. Chandrashekar, Sandeep Gupta, Surya Prakash, Lalji Singh

Research output: Contribution to journalReview articlepeer-review

19 Scopus citations


Wild animals are an integral component of the ecosystem. Their decimation due to abrupt natural calamities or due to gradual human intervention would be disastrous to the ecosystem and would alter the balance in nature between various biotic components. Such an imbalance could have an adverse effects on the ecosystem. Therefore, there is an urgent need to put an end to the ever increasing list of endangered species by undertaking both in situ and ex situ conservation using tools of modern biology, to ascertain the degree of genetic variation and reproductive competence in these animals. This review highlights the development and use of molecular markers such as microsatellites, minisatellites, mitochondrial control region, cytochrome b and MHC loci to assess the genetic variation in various Indian wild animals such as the lion, tiger, leopard and deer. The review also presents data on the semen profile of the big cats of India. Reproductive technologies such as cryopreservation of semen and artificial insemination in big cats are also highlighted.

Original languageEnglish (US)
Pages (from-to)710-723
Number of pages14
JournalIndian Journal of Experimental Biology
Issue number7
StatePublished - Jul 1 2003
Externally publishedYes


  • Artificial insemination
  • Cryopreservation
  • MHC
  • Microsatellite
  • SNPs
  • Semen profile
  • mt DNA

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology


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