Comparative study of regenerative effects of mesenchymal stem cells derived from placental amnion, chorion and umbilical cord on dermal wounds

Juliane Ertl; Melanie Pichlsberger; Alexandru Cristian Tuca; Paul Wurzer; Jakob Fuchs; Stefan H. Geyer; Barbara Maurer-Gesek; Wolfgang J. Weninger; Dagmar Pfeiffer; Vladimir Bubalo; Daryousch Parvizi; Lars Peter Kamolz; Ingrid Lang

doi:10.1016/j.placenta.2018.04.004

Comparative study of regenerative effects of mesenchymal stem cells derived from placental amnion, chorion and umbilical cord on dermal wounds

Juliane Ertl, Melanie Pichlsberger, Alexandru Cristian Tuca, Paul Wurzer, Jakob Fuchs, Stefan H. Geyer, Barbara Maurer-Gesek, Wolfgang J. Weninger, Dagmar Pfeiffer, Vladimir Bubalo, Daryousch Parvizi, Lars Peter Kamolz, Ingrid Lang

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Objective: Mesenchymal stem/stromal cells derived from human term placentas (PMSCs) are novel therapeutic agents and more topical than ever. Here we evaluated the effects of three types of PMSCs on wound healing in an in vivo mouse model: Amnion-derived MSCs (AMSCs), blood vessel-derived MSCs (BV-MSCs) from the chorionic plate and Wharton's jelly-derived MSCs (WJ-MSCs) from the umbilical cord. Methods: We topically applied PMSCs onto skin wounds in mice using the dermal substitute Matriderm ^® as carrier and evaluated wound healing parameters. In addition, we investigated the effects of all PMSC types under co-application with placental endothelial cells (PLECs). After 8 days, we compared the percent of wound closure and the angiogenic potential between all groups. Results: AMSCs, BV-MSCs and WJ-MSCs significantly induced a faster healing and a higher number of blood vessels in the wound when compared to controls (Matriderm ^® -alone). PLECs did not further improve the advantageous effects of PMSC-treatment. Quantitative data and 3D analysis by high resolution episcopic microscopy confirmed a lower density of vessels in Matriderm ^® /PMSCs/PLECs co-application compared to Matriderm ^® /PMSCs treatment. Conclusion: Results indicate that all three PMSC types exert similar beneficial effects on wound closure and neovascularization in our mouse model. Practice: Using Matriderm ^® as carrier for PMSCs propagates rapid cell migration towards the wound area that allows a fast and clinically practicable method for stem cell application. Implications: These promising effects warrant further investigation in clinical trials.

Original language	English (US)
Pages (from-to)	37-46
Number of pages	10
Journal	Placenta
Volume	65
DOIs	https://doi.org/10.1016/j.placenta.2018.04.004
State	Published - May 2018
Externally published	Yes

Keywords

Amnion
Angiogenesis
Blood vessels
Human placenta
Mesenchymal stem cells
Mouse model
Umbilical cord
Wound healing

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology
Developmental Biology

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10.1016/j.placenta.2018.04.004

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Ertl, J., Pichlsberger, M., Tuca, A. C., Wurzer, P., Fuchs, J., Geyer, S. H., Maurer-Gesek, B., Weninger, W. J., Pfeiffer, D., Bubalo, V., Parvizi, D., Kamolz, L. P., & Lang, I. (2018). Comparative study of regenerative effects of mesenchymal stem cells derived from placental amnion, chorion and umbilical cord on dermal wounds. Placenta, 65, 37-46. https://doi.org/10.1016/j.placenta.2018.04.004

Ertl, J, Pichlsberger, M, Tuca, AC, Wurzer, P, Fuchs, J, Geyer, SH, Maurer-Gesek, B, Weninger, WJ, Pfeiffer, D, Bubalo, V, Parvizi, D, Kamolz, LP & Lang, I 2018, 'Comparative study of regenerative effects of mesenchymal stem cells derived from placental amnion, chorion and umbilical cord on dermal wounds', Placenta, vol. 65, pp. 37-46. https://doi.org/10.1016/j.placenta.2018.04.004

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title = "Comparative study of regenerative effects of mesenchymal stem cells derived from placental amnion, chorion and umbilical cord on dermal wounds",

abstract = " Objective: Mesenchymal stem/stromal cells derived from human term placentas (PMSCs) are novel therapeutic agents and more topical than ever. Here we evaluated the effects of three types of PMSCs on wound healing in an in vivo mouse model: Amnion-derived MSCs (AMSCs), blood vessel-derived MSCs (BV-MSCs) from the chorionic plate and Wharton's jelly-derived MSCs (WJ-MSCs) from the umbilical cord. Methods: We topically applied PMSCs onto skin wounds in mice using the dermal substitute Matriderm {\textregistered} as carrier and evaluated wound healing parameters. In addition, we investigated the effects of all PMSC types under co-application with placental endothelial cells (PLECs). After 8 days, we compared the percent of wound closure and the angiogenic potential between all groups. Results: AMSCs, BV-MSCs and WJ-MSCs significantly induced a faster healing and a higher number of blood vessels in the wound when compared to controls (Matriderm {\textregistered} -alone). PLECs did not further improve the advantageous effects of PMSC-treatment. Quantitative data and 3D analysis by high resolution episcopic microscopy confirmed a lower density of vessels in Matriderm {\textregistered} /PMSCs/PLECs co-application compared to Matriderm {\textregistered} /PMSCs treatment. Conclusion: Results indicate that all three PMSC types exert similar beneficial effects on wound closure and neovascularization in our mouse model. Practice: Using Matriderm {\textregistered} as carrier for PMSCs propagates rapid cell migration towards the wound area that allows a fast and clinically practicable method for stem cell application. Implications: These promising effects warrant further investigation in clinical trials.",

keywords = "Amnion, Angiogenesis, Blood vessels, Human placenta, Mesenchymal stem cells, Mouse model, Umbilical cord, Wound healing",

author = "Juliane Ertl and Melanie Pichlsberger and Tuca, {Alexandru Cristian} and Paul Wurzer and Jakob Fuchs and Geyer, {Stefan H.} and Barbara Maurer-Gesek and Weninger, {Wolfgang J.} and Dagmar Pfeiffer and Vladimir Bubalo and Daryousch Parvizi and Kamolz, {Lars Peter} and Ingrid Lang",

note = "Publisher Copyright: {\textcopyright} 2018",

year = "2018",

month = may,

doi = "10.1016/j.placenta.2018.04.004",

language = "English (US)",

volume = "65",

pages = "37--46",

journal = "Placenta",

issn = "0143-4004",

publisher = "W.B. Saunders Ltd",

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TY - JOUR

T1 - Comparative study of regenerative effects of mesenchymal stem cells derived from placental amnion, chorion and umbilical cord on dermal wounds

AU - Ertl, Juliane

AU - Pichlsberger, Melanie

AU - Tuca, Alexandru Cristian

AU - Wurzer, Paul

AU - Fuchs, Jakob

AU - Geyer, Stefan H.

AU - Maurer-Gesek, Barbara

AU - Weninger, Wolfgang J.

AU - Pfeiffer, Dagmar

AU - Bubalo, Vladimir

AU - Parvizi, Daryousch

AU - Kamolz, Lars Peter

AU - Lang, Ingrid

PY - 2018/5

Y1 - 2018/5

N2 - Objective: Mesenchymal stem/stromal cells derived from human term placentas (PMSCs) are novel therapeutic agents and more topical than ever. Here we evaluated the effects of three types of PMSCs on wound healing in an in vivo mouse model: Amnion-derived MSCs (AMSCs), blood vessel-derived MSCs (BV-MSCs) from the chorionic plate and Wharton's jelly-derived MSCs (WJ-MSCs) from the umbilical cord. Methods: We topically applied PMSCs onto skin wounds in mice using the dermal substitute Matriderm ® as carrier and evaluated wound healing parameters. In addition, we investigated the effects of all PMSC types under co-application with placental endothelial cells (PLECs). After 8 days, we compared the percent of wound closure and the angiogenic potential between all groups. Results: AMSCs, BV-MSCs and WJ-MSCs significantly induced a faster healing and a higher number of blood vessels in the wound when compared to controls (Matriderm ® -alone). PLECs did not further improve the advantageous effects of PMSC-treatment. Quantitative data and 3D analysis by high resolution episcopic microscopy confirmed a lower density of vessels in Matriderm ® /PMSCs/PLECs co-application compared to Matriderm ® /PMSCs treatment. Conclusion: Results indicate that all three PMSC types exert similar beneficial effects on wound closure and neovascularization in our mouse model. Practice: Using Matriderm ® as carrier for PMSCs propagates rapid cell migration towards the wound area that allows a fast and clinically practicable method for stem cell application. Implications: These promising effects warrant further investigation in clinical trials.

AB - Objective: Mesenchymal stem/stromal cells derived from human term placentas (PMSCs) are novel therapeutic agents and more topical than ever. Here we evaluated the effects of three types of PMSCs on wound healing in an in vivo mouse model: Amnion-derived MSCs (AMSCs), blood vessel-derived MSCs (BV-MSCs) from the chorionic plate and Wharton's jelly-derived MSCs (WJ-MSCs) from the umbilical cord. Methods: We topically applied PMSCs onto skin wounds in mice using the dermal substitute Matriderm ® as carrier and evaluated wound healing parameters. In addition, we investigated the effects of all PMSC types under co-application with placental endothelial cells (PLECs). After 8 days, we compared the percent of wound closure and the angiogenic potential between all groups. Results: AMSCs, BV-MSCs and WJ-MSCs significantly induced a faster healing and a higher number of blood vessels in the wound when compared to controls (Matriderm ® -alone). PLECs did not further improve the advantageous effects of PMSC-treatment. Quantitative data and 3D analysis by high resolution episcopic microscopy confirmed a lower density of vessels in Matriderm ® /PMSCs/PLECs co-application compared to Matriderm ® /PMSCs treatment. Conclusion: Results indicate that all three PMSC types exert similar beneficial effects on wound closure and neovascularization in our mouse model. Practice: Using Matriderm ® as carrier for PMSCs propagates rapid cell migration towards the wound area that allows a fast and clinically practicable method for stem cell application. Implications: These promising effects warrant further investigation in clinical trials.

KW - Amnion

KW - Angiogenesis

KW - Blood vessels

KW - Human placenta

KW - Mesenchymal stem cells

KW - Mouse model

KW - Umbilical cord

KW - Wound healing

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U2 - 10.1016/j.placenta.2018.04.004

DO - 10.1016/j.placenta.2018.04.004

M3 - Article

AN - SCOPUS:85045283245

SN - 0143-4004

VL - 65

SP - 37

EP - 46

JO - Placenta

JF - Placenta

ER -

Comparative study of regenerative effects of mesenchymal stem cells derived from placental amnion, chorion and umbilical cord on dermal wounds

Abstract

Keywords

ASJC Scopus subject areas

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