Combination of widefield fluorescence imaging and nonlinear optical microscopy of oral epithelial neoplasia

Rahul Pal, Kert Edward, Tyra Brown, Liang Ma, Jinping Yang, Susan McCammon, Massoud Motamedi, Gracie Vargas

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Multiphoton Autofluorescence Microscopy (MPAM) and Second Harmonic Generation Microscopy (SHGM) have shown the potential for noninvasive assessment of oral precancers and cancers. We have explored a combination of these nonlinear optical microscopic imaging techniques with widefield fluorescence imaging to assess morphometry similar to that of pathologic evaluation as well as information from endogenous fluorophores, which are altered with neoplastic transformation. Widefield fluorescence revealed areas of interest corresponding to sites with precancers or early tumors, generally resulting in a decrease in green emission or increase in red emission. Subsequent microscopy revealed significant differences in morphology between normal, dysplastic/neoplastic mucosa for all layers. Combination of a widefield and a microscopic technique provides a novel approach for tissue morphometric analysis along with large area assessment of tissue autofluorescence properties.

Original languageEnglish (US)
Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems XI
StatePublished - 2013
EventAdvanced Biomedical and Clinical Diagnostic Systems XI - San Francisco, CA, United States
Duration: Feb 3 2013Feb 5 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherAdvanced Biomedical and Clinical Diagnostic Systems XI
Country/TerritoryUnited States
CitySan Francisco, CA


  • Multiphoton microscopy
  • oral precancer
  • second harmonic generation microscopy
  • wide-field fluorescence imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


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