Colloidal gold labeling of sections and cell surfaces

Hal K. Hawkins; Linda S. Rehm; Jessica Y. Zhu

doi:10.3109/01913129209074551

Colloidal gold labeling of sections and cell surfaces

Hal K. Hawkins, Linda S. Rehm, Jessica Y. Zhu

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Colloidal gold labeling techniques can be simplified enough to become practical for diagnostic use in a hospital electron microscopy laboratory. Methods found to be practical and reproducible are presented, including production of colloidal gold reagents, polar embedding for gold labeling of thin sections, and use of silver enhancement on semithin sections. Methods for cell surface labeling are emphasized; they are technically straightforward and can be applied to investigation of multiple diagnostic problems.

Original language	English (US)
Pages (from-to)	61-70
Number of pages	10
Journal	Ultrastructural Pathology
Volume	16
Issue number	1-2
DOIs	https://doi.org/10.3109/01913129209074551
State	Published - 1992
Externally published	Yes

Keywords

Adhesion molecules
Colloidal gold
Immunohistochemistry
Mac-1
Methods
Receptors

ASJC Scopus subject areas

Pathology and Forensic Medicine
Structural Biology

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10.3109/01913129209074551

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title = "Colloidal gold labeling of sections and cell surfaces",

abstract = "Colloidal gold labeling techniques can be simplified enough to become practical for diagnostic use in a hospital electron microscopy laboratory. Methods found to be practical and reproducible are presented, including production of colloidal gold reagents, polar embedding for gold labeling of thin sections, and use of silver enhancement on semithin sections. Methods for cell surface labeling are emphasized; they are technically straightforward and can be applied to investigation of multiple diagnostic problems.",

keywords = "Adhesion molecules, Colloidal gold, Immunohistochemistry, Mac-1, Methods, Receptors",

author = "Hawkins, {Hal K.} and Rehm, {Linda S.} and Zhu, {Jessica Y.}",

year = "1992",

doi = "10.3109/01913129209074551",

language = "English (US)",

volume = "16",

pages = "61--70",

journal = "Ultrastructural Pathology",

issn = "0191-3123",

publisher = "Informa Healthcare",

number = "1-2",

}

TY - JOUR

T1 - Colloidal gold labeling of sections and cell surfaces

AU - Hawkins, Hal K.

AU - Rehm, Linda S.

AU - Zhu, Jessica Y.

PY - 1992

Y1 - 1992

N2 - Colloidal gold labeling techniques can be simplified enough to become practical for diagnostic use in a hospital electron microscopy laboratory. Methods found to be practical and reproducible are presented, including production of colloidal gold reagents, polar embedding for gold labeling of thin sections, and use of silver enhancement on semithin sections. Methods for cell surface labeling are emphasized; they are technically straightforward and can be applied to investigation of multiple diagnostic problems.

AB - Colloidal gold labeling techniques can be simplified enough to become practical for diagnostic use in a hospital electron microscopy laboratory. Methods found to be practical and reproducible are presented, including production of colloidal gold reagents, polar embedding for gold labeling of thin sections, and use of silver enhancement on semithin sections. Methods for cell surface labeling are emphasized; they are technically straightforward and can be applied to investigation of multiple diagnostic problems.

KW - Adhesion molecules

KW - Colloidal gold

KW - Immunohistochemistry

KW - Mac-1

KW - Methods

KW - Receptors

UR - http://www.scopus.com/inward/record.url?scp=0026530227&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026530227&partnerID=8YFLogxK

U2 - 10.3109/01913129209074551

DO - 10.3109/01913129209074551

M3 - Article

C2 - 1557831

AN - SCOPUS:0026530227

SN - 0191-3123

VL - 16

SP - 61

EP - 70

JO - Ultrastructural Pathology

JF - Ultrastructural Pathology

IS - 1-2

ER -

Colloidal gold labeling of sections and cell surfaces

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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