Abstract
According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 106-108 |
| Number of pages | 3 |
| Journal | Sheng wu gong cheng xue bao = Chinese journal of biotechnology |
| Volume | 18 |
| Issue number | 1 |
| State | Published - Jan 2002 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology