Cloning and characterization of a histone gene family in Tilapia fish

Ella Englander; Boaz Moav

doi:10.1016/0167-4781(89)90148-6

Cloning and characterization of a histone gene family in Tilapia fish

Ella Englander, Boaz Moav

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Genomic organization and expression of a histone gene family in the Tilapia (Cichlidae) fish was determined. A genomic library was prepared; four clones containing the complete set of core histones were analyzed. These clones differed significantly in their restriction maps, although three of them revealed a uniform internai gene arrangement of the core histone genes. Differential expression between two of the cloned clusters was observed when the hybridization pattern of those clusters to various RNA preparations was compared. The copy number of histone genes was estimated to be 100 to 110 for haploid Tilapia genome.

Original language	English (US)
Pages (from-to)	277-282
Number of pages	6
Journal	BBA - Gene Structure and Expression
Volume	1007
Issue number	3
DOIs	https://doi.org/10.1016/0167-4781(89)90148-6
State	Published - Apr 12 1989
Externally published	Yes

Keywords

(Tilapia fish)
Gene characterization
Gene cloning
Genomic organization
Histone gene family

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(89)90148-6

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abstract = "Genomic organization and expression of a histone gene family in the Tilapia (Cichlidae) fish was determined. A genomic library was prepared; four clones containing the complete set of core histones were analyzed. These clones differed significantly in their restriction maps, although three of them revealed a uniform internai gene arrangement of the core histone genes. Differential expression between two of the cloned clusters was observed when the hybridization pattern of those clusters to various RNA preparations was compared. The copy number of histone genes was estimated to be 100 to 110 for haploid Tilapia genome.",

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T1 - Cloning and characterization of a histone gene family in Tilapia fish

AU - Englander, Ella

AU - Moav, Boaz

PY - 1989/4/12

Y1 - 1989/4/12

N2 - Genomic organization and expression of a histone gene family in the Tilapia (Cichlidae) fish was determined. A genomic library was prepared; four clones containing the complete set of core histones were analyzed. These clones differed significantly in their restriction maps, although three of them revealed a uniform internai gene arrangement of the core histone genes. Differential expression between two of the cloned clusters was observed when the hybridization pattern of those clusters to various RNA preparations was compared. The copy number of histone genes was estimated to be 100 to 110 for haploid Tilapia genome.

AB - Genomic organization and expression of a histone gene family in the Tilapia (Cichlidae) fish was determined. A genomic library was prepared; four clones containing the complete set of core histones were analyzed. These clones differed significantly in their restriction maps, although three of them revealed a uniform internai gene arrangement of the core histone genes. Differential expression between two of the cloned clusters was observed when the hybridization pattern of those clusters to various RNA preparations was compared. The copy number of histone genes was estimated to be 100 to 110 for haploid Tilapia genome.

KW - (Tilapia fish)

KW - Gene characterization

KW - Gene cloning

KW - Genomic organization

KW - Histone gene family

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VL - 1007

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EP - 282

JO - BBA - Gene Structure and Expression

JF - BBA - Gene Structure and Expression

IS - 3

ER -

Cloning and characterization of a histone gene family in Tilapia fish

Abstract

Keywords

ASJC Scopus subject areas

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