Class II MHC-expressing myofibroblasts play a role in the immunopathogenesis associated with staphylococcal enterotoxins

C. A. Barrera; I. V. Pinchuk; J. I. Saada; G. Suarez; D. A. Bland; E. Beswick; P. A. Adegboyega; R. C. Mifflin; D. W. Powell; V. E. Reyes

doi:10.1196/annals.1309.022

Class II MHC-expressing myofibroblasts play a role in the immunopathogenesis associated with staphylococcal enterotoxins

C. A. Barrera, I. V. Pinchuk, J. I. Saada, G. Suarez, D. A. Bland, E. Beswick, P. A. Adegboyega, R. C. Mifflin, D. W. Powell, V. E. Reyes

Pediatrics

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Food poisoning due to staphylococcal enterotoxins (SEs) affects hundreds of thousands of people each year. Little is known about how SEs initiate immune responses and cause pathogenesis. Here, we demonstrate that cultured human intestinal myofibroblasts (IMFs) bind SEs in an MHC class II-dependent fashion. IMFs respond to SE exposure with increased secretion of IL-6, IL-8, and TNF-α. A significant proliferative T cell response was observed when MHC class II-expressing IMFs were pulsed with SEA and cocultured with human CD4 ⁺ T cells. In conclusion, our findings support the hypothesis that IMFs may play an important role in pathology associated with staphlococcocal enterotoxigenic disease.

Original language	English (US)
Pages (from-to)	313-318
Number of pages	6
Journal	Annals of the New York Academy of Sciences
Volume	1029
DOIs	https://doi.org/10.1196/annals.1309.022
State	Published - 2004

Keywords

Cytokines
MHC class II molecule
Myofibroblast
Staphylococcal enterotoxins
Superantigen

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
History and Philosophy of Science

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Barrera, C. A., Pinchuk, I. V., Saada, J. I., Suarez, G., Bland, D. A., Beswick, E., Adegboyega, P. A., Mifflin, R. C., Powell, D. W., & Reyes, V. E. (2004). Class II MHC-expressing myofibroblasts play a role in the immunopathogenesis associated with staphylococcal enterotoxins. Annals of the New York Academy of Sciences, 1029, 313-318. https://doi.org/10.1196/annals.1309.022

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title = "Class II MHC-expressing myofibroblasts play a role in the immunopathogenesis associated with staphylococcal enterotoxins",

abstract = "Food poisoning due to staphylococcal enterotoxins (SEs) affects hundreds of thousands of people each year. Little is known about how SEs initiate immune responses and cause pathogenesis. Here, we demonstrate that cultured human intestinal myofibroblasts (IMFs) bind SEs in an MHC class II-dependent fashion. IMFs respond to SE exposure with increased secretion of IL-6, IL-8, and TNF-α. A significant proliferative T cell response was observed when MHC class II-expressing IMFs were pulsed with SEA and cocultured with human CD4 + T cells. In conclusion, our findings support the hypothesis that IMFs may play an important role in pathology associated with staphlococcocal enterotoxigenic disease.",

keywords = "Cytokines, MHC class II molecule, Myofibroblast, Staphylococcal enterotoxins, Superantigen",

author = "Barrera, {C. A.} and Pinchuk, {I. V.} and Saada, {J. I.} and G. Suarez and Bland, {D. A.} and E. Beswick and Adegboyega, {P. A.} and Mifflin, {R. C.} and Powell, {D. W.} and Reyes, {V. E.}",

year = "2004",

doi = "10.1196/annals.1309.022",

language = "English (US)",

volume = "1029",

pages = "313--318",

journal = "Annals of the New York Academy of Sciences",

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TY - JOUR

T1 - Class II MHC-expressing myofibroblasts play a role in the immunopathogenesis associated with staphylococcal enterotoxins

AU - Barrera, C. A.

AU - Pinchuk, I. V.

AU - Saada, J. I.

AU - Suarez, G.

AU - Bland, D. A.

AU - Beswick, E.

AU - Adegboyega, P. A.

AU - Mifflin, R. C.

AU - Powell, D. W.

AU - Reyes, V. E.

PY - 2004

Y1 - 2004

N2 - Food poisoning due to staphylococcal enterotoxins (SEs) affects hundreds of thousands of people each year. Little is known about how SEs initiate immune responses and cause pathogenesis. Here, we demonstrate that cultured human intestinal myofibroblasts (IMFs) bind SEs in an MHC class II-dependent fashion. IMFs respond to SE exposure with increased secretion of IL-6, IL-8, and TNF-α. A significant proliferative T cell response was observed when MHC class II-expressing IMFs were pulsed with SEA and cocultured with human CD4 + T cells. In conclusion, our findings support the hypothesis that IMFs may play an important role in pathology associated with staphlococcocal enterotoxigenic disease.

AB - Food poisoning due to staphylococcal enterotoxins (SEs) affects hundreds of thousands of people each year. Little is known about how SEs initiate immune responses and cause pathogenesis. Here, we demonstrate that cultured human intestinal myofibroblasts (IMFs) bind SEs in an MHC class II-dependent fashion. IMFs respond to SE exposure with increased secretion of IL-6, IL-8, and TNF-α. A significant proliferative T cell response was observed when MHC class II-expressing IMFs were pulsed with SEA and cocultured with human CD4 + T cells. In conclusion, our findings support the hypothesis that IMFs may play an important role in pathology associated with staphlococcocal enterotoxigenic disease.

KW - Cytokines

KW - MHC class II molecule

KW - Myofibroblast

KW - Staphylococcal enterotoxins

KW - Superantigen

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U2 - 10.1196/annals.1309.022

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SN - 0077-8923

VL - 1029

SP - 313

EP - 318

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

ER -

Class II MHC-expressing myofibroblasts play a role in the immunopathogenesis associated with staphylococcal enterotoxins

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Keywords

ASJC Scopus subject areas

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