Chromosomal DNA contains the gene coding for Salmonella enterotoxin

A. K. Chopra; C. W. Houston; J. W. Peterson; J. J. Mekalanos

doi:10.1111/j.1574-6968.1987.tb02170.x

Chromosomal DNA contains the gene coding for Salmonella enterotoxin

A. K. Chopra, C. W. Houston, J. W. Peterson, J. J. Mekalanos

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

This report examines the genetic basis for Salmonella enterotoxin production. The examination was conducted using an internal XbaI/HincII sequence of plasmid pJM17 encoding cholera toxin as a gene probe (ctxAB). This gene probe detected some sequence homology with the total as well as digested chromosomal DNA fragments from Salmonella strains under reduced stringent conditions in dot-blot and Southern-blot hybridization experiments. Our hybridization analysis results suggested that the enterotoxin (ent) gene of Salmonella resides on chromosomal DNA and that the Salmonella ent operon might be duplicated on the Salmonella chromosome.

Original language	English (US)
Pages (from-to)	345-349
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	43
Issue number	3
DOIs	https://doi.org/10.1111/j.1574-6968.1987.tb02170.x
State	Published - Sep 1987

Keywords

(Southern-blot hybridization)
Salmonella enterotoxin
cholera toxin
chromosomal gene

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

Access to Document

10.1111/j.1574-6968.1987.tb02170.x

Cite this

@article{009bdc5cd9a949a29b7983294db4f40b,

title = "Chromosomal DNA contains the gene coding for Salmonella enterotoxin",

abstract = "This report examines the genetic basis for Salmonella enterotoxin production. The examination was conducted using an internal XbaI/HincII sequence of plasmid pJM17 encoding cholera toxin as a gene probe (ctxAB). This gene probe detected some sequence homology with the total as well as digested chromosomal DNA fragments from Salmonella strains under reduced stringent conditions in dot-blot and Southern-blot hybridization experiments. Our hybridization analysis results suggested that the enterotoxin (ent) gene of Salmonella resides on chromosomal DNA and that the Salmonella ent operon might be duplicated on the Salmonella chromosome.",

keywords = "(Southern-blot hybridization), Salmonella enterotoxin, cholera toxin, chromosomal gene",

author = "Chopra, {A. K.} and Houston, {C. W.} and Peterson, {J. W.} and Mekalanos, {J. J.}",

note = "Funding Information: ease Control, Atlanta, GA; E.S. Anderson, Enteric Disease Reference Laboratory, Colinsdale, U.K.; and Q.T. Box, Department of Pediatrics and Microbiology, Galveston, TX for providing us Salmonella strains. Vibrio cholerae 569B was provided to us by H.L. Smith, Thomas Jefferson University, Philadelphia, PA. This investigation was supported in part by Public Health Service Grants 1 R23 AI 21075 and 1 R01 AI 18401 from the National Institutes of Health awarded to C.W. Houston and J.W. Peter-son, respectively. A.K. Chopra is a McLaughlin Postdoctoral fellow.",

year = "1987",

month = sep,

doi = "10.1111/j.1574-6968.1987.tb02170.x",

language = "English (US)",

volume = "43",

pages = "345--349",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Chromosomal DNA contains the gene coding for Salmonella enterotoxin

AU - Chopra, A. K.

AU - Houston, C. W.

AU - Peterson, J. W.

AU - Mekalanos, J. J.

N1 - Funding Information: ease Control, Atlanta, GA; E.S. Anderson, Enteric Disease Reference Laboratory, Colinsdale, U.K.; and Q.T. Box, Department of Pediatrics and Microbiology, Galveston, TX for providing us Salmonella strains. Vibrio cholerae 569B was provided to us by H.L. Smith, Thomas Jefferson University, Philadelphia, PA. This investigation was supported in part by Public Health Service Grants 1 R23 AI 21075 and 1 R01 AI 18401 from the National Institutes of Health awarded to C.W. Houston and J.W. Peter-son, respectively. A.K. Chopra is a McLaughlin Postdoctoral fellow.

PY - 1987/9

Y1 - 1987/9

N2 - This report examines the genetic basis for Salmonella enterotoxin production. The examination was conducted using an internal XbaI/HincII sequence of plasmid pJM17 encoding cholera toxin as a gene probe (ctxAB). This gene probe detected some sequence homology with the total as well as digested chromosomal DNA fragments from Salmonella strains under reduced stringent conditions in dot-blot and Southern-blot hybridization experiments. Our hybridization analysis results suggested that the enterotoxin (ent) gene of Salmonella resides on chromosomal DNA and that the Salmonella ent operon might be duplicated on the Salmonella chromosome.

AB - This report examines the genetic basis for Salmonella enterotoxin production. The examination was conducted using an internal XbaI/HincII sequence of plasmid pJM17 encoding cholera toxin as a gene probe (ctxAB). This gene probe detected some sequence homology with the total as well as digested chromosomal DNA fragments from Salmonella strains under reduced stringent conditions in dot-blot and Southern-blot hybridization experiments. Our hybridization analysis results suggested that the enterotoxin (ent) gene of Salmonella resides on chromosomal DNA and that the Salmonella ent operon might be duplicated on the Salmonella chromosome.

KW - (Southern-blot hybridization)

KW - Salmonella enterotoxin

KW - cholera toxin

KW - chromosomal gene

UR - http://www.scopus.com/inward/record.url?scp=38249034297&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38249034297&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6968.1987.tb02170.x

DO - 10.1111/j.1574-6968.1987.tb02170.x

M3 - Article

AN - SCOPUS:38249034297

SN - 0378-1097

VL - 43

SP - 345

EP - 349

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 3

ER -

Chromosomal DNA contains the gene coding for Salmonella enterotoxin

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this