TY - JOUR
T1 - Chemokines are present in the genital tract of HIV-seropositive and HIV-seronegative women
T2 - Correlation with other immune mediators
AU - Spear, Gregory T.
AU - Sha, Beverly E.
AU - Saarloos, Mary Nel
AU - Benson, Constance A.
AU - Rydman, Robert
AU - Massad, L. Stewart
AU - Gilmore, Ruth
AU - Landay, Alan L.
PY - 1998/8/15
Y1 - 1998/8/15
N2 - In this cross-sectional study, 53 cervicovaginal lavage samples (CVL) from 41 women were analyzed for the chemokines interleukin-8 (IL-8), regulated-on-activation normal T-expressed and secreted (RANTES) factor, and macrophage inflammatory protein-1α (MIP-1α) by enzyme-linked immunosorbent assay (ELISA). IL-8 was detected in 81% of CVL, whereas RANTES was detected in 32%, and MIP-1α in 15% of the CVL. The mean levels of IL-8, RANTES, and MIP-1α in positive samples were 396 pg/ml, 102 pg/ml, and 34 pg/ml, respectively. IL-8 levels correlated positively with IL-1β and IgG in a subset of CVL samples. RANTES levels correlated positively with complement protein levels. Additionally, the levels of RANTES but not MIP-1α, reached levels reported in previous studies of the effects of β chemokines to inhibit HIV replication. These results suggest that measuring chemokines in CVL specimens can provide important information regarding immune responses in the genital tract.
AB - In this cross-sectional study, 53 cervicovaginal lavage samples (CVL) from 41 women were analyzed for the chemokines interleukin-8 (IL-8), regulated-on-activation normal T-expressed and secreted (RANTES) factor, and macrophage inflammatory protein-1α (MIP-1α) by enzyme-linked immunosorbent assay (ELISA). IL-8 was detected in 81% of CVL, whereas RANTES was detected in 32%, and MIP-1α in 15% of the CVL. The mean levels of IL-8, RANTES, and MIP-1α in positive samples were 396 pg/ml, 102 pg/ml, and 34 pg/ml, respectively. IL-8 levels correlated positively with IL-1β and IgG in a subset of CVL samples. RANTES levels correlated positively with complement protein levels. Additionally, the levels of RANTES but not MIP-1α, reached levels reported in previous studies of the effects of β chemokines to inhibit HIV replication. These results suggest that measuring chemokines in CVL specimens can provide important information regarding immune responses in the genital tract.
KW - Cervicovaginal lavage
KW - Chemokine
KW - Genital tract
KW - HIV-1
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U2 - 10.1097/00042560-199808150-00006
DO - 10.1097/00042560-199808150-00006
M3 - Article
C2 - 9715841
AN - SCOPUS:0032529232
SN - 1077-9450
VL - 18
SP - 454
EP - 459
JO - Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
JF - Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
IS - 5
ER -