Characterization of the yehUT two-component regulatory system of Salmonella enterica serovar Typhi and Typhimurium

Vanessa K. Wong; Derek J. Pickard; Lars Barquist; Karthikeyan Sivaraman; Andrew J. Page; Peter J. Hart; Mark J. Arends; Kathryn E. Holt; Leanne Kane; Lynda F. Mottram; Louise Ellison; Ruben Bautista; Chris J. McGee; Sally J. Kay; Thomas M. Wileman; Linda J. Kenney; Calman A. MacLennan; Robert A. Kingsley; Gordon Dougan

doi:10.1371/journal.pone.0084567

Characterization of the yehUT two-component regulatory system of Salmonella enterica serovar Typhi and Typhimurium

Vanessa K. Wong, Derek J. Pickard, Lars Barquist, Karthikeyan Sivaraman, Andrew J. Page, Peter J. Hart, Mark J. Arends, Kathryn E. Holt, Leanne Kane, Lynda F. Mottram, Louise Ellison, Ruben Bautista, Chris J. McGee, Sally J. Kay, Thomas M. Wileman, Linda J. Kenney, Calman A. MacLennan, Robert A. Kingsley, Gordon Dougan

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Abstract

Proteins exhibiting hyper-variable sequences within a bacterial pathogen may be associated with host adaptation. Several lineages of the monophyletic pathogen Salmonella enterica serovar Typhi (S. Typhi) have accumulated non-synonymous mutations in the putative two-component regulatory system yehUT. Consequently we evaluated the function of yehUT in S. Typhi BRD948 and S. Typhimurium ST4/74. Transcriptome analysis identified the cstA gene, encoding a carbon starvation protein as the predominantly yehUT regulated gene in both these serovars. Deletion of yehUT had no detectable effect on the ability of these mutant Salmonella to invade cultured epithelial cells (S. Typhi and S. Typhimurium) or induce colitis in a murine model (S. Typhimurium only). Growth, metabolic and antimicrobial susceptibility tests identified no obvious influences of yehUT on these phenotypes.

Original language	English (US)
Article number	e84567
Journal	PloS one
Volume	8
Issue number	12
DOIs	https://doi.org/10.1371/journal.pone.0084567
State	Published - Dec 30 2013
Externally published	Yes

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Wong, V. K., Pickard, D. J., Barquist, L., Sivaraman, K., Page, A. J., Hart, P. J., Arends, M. J., Holt, K. E., Kane, L., Mottram, L. F., Ellison, L., Bautista, R., McGee, C. J., Kay, S. J., Wileman, T. M., Kenney, L. J., MacLennan, C. A., Kingsley, R. A., & Dougan, G. (2013). Characterization of the yehUT two-component regulatory system of Salmonella enterica serovar Typhi and Typhimurium. PloS one, 8(12), Article e84567. https://doi.org/10.1371/journal.pone.0084567

Wong, VK, Pickard, DJ, Barquist, L, Sivaraman, K, Page, AJ, Hart, PJ, Arends, MJ, Holt, KE, Kane, L, Mottram, LF, Ellison, L, Bautista, R, McGee, CJ, Kay, SJ, Wileman, TM, Kenney, LJ, MacLennan, CA, Kingsley, RA & Dougan, G 2013, 'Characterization of the yehUT two-component regulatory system of Salmonella enterica serovar Typhi and Typhimurium', PloS one, vol. 8, no. 12, e84567. https://doi.org/10.1371/journal.pone.0084567

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abstract = "Proteins exhibiting hyper-variable sequences within a bacterial pathogen may be associated with host adaptation. Several lineages of the monophyletic pathogen Salmonella enterica serovar Typhi (S. Typhi) have accumulated non-synonymous mutations in the putative two-component regulatory system yehUT. Consequently we evaluated the function of yehUT in S. Typhi BRD948 and S. Typhimurium ST4/74. Transcriptome analysis identified the cstA gene, encoding a carbon starvation protein as the predominantly yehUT regulated gene in both these serovars. Deletion of yehUT had no detectable effect on the ability of these mutant Salmonella to invade cultured epithelial cells (S. Typhi and S. Typhimurium) or induce colitis in a murine model (S. Typhimurium only). Growth, metabolic and antimicrobial susceptibility tests identified no obvious influences of yehUT on these phenotypes.",

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AU - Arends, Mark J.

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AU - Dougan, Gordon

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Characterization of the yehUT two-component regulatory system of Salmonella enterica serovar Typhi and Typhimurium

Abstract

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