Characterization of the high pH wobble structure of the 2-aminopurine·cytosine mismatch by N-15 NMR spectroscopy

Lawrence C. Sowers; Ramon Eritja; Fu Ming Chen; Tasneem Khwaja; Bruce E. Kaplan; Myron F. Goodman; G. Victor Fazakerley

doi:10.1016/0006-291X(89)91037-1

Characterization of the high pH wobble structure of the 2-aminopurine·cytosine mismatch by N-15 NMR spectroscopy

Lawrence C. Sowers, Ramon Eritja, Fu Ming Chen, Tasneem Khwaja, Bruce E. Kaplan, Myron F. Goodman, G. Victor Fazakerley

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Transition mutations induced by the base analogue 2-aminopurine arise via the formation of AP·C base pairs during DNA replication. We report here the results of N-15 NMR studies on a duplex oligonucleotide containing N-15 enriched AP and C residues. At high pH (8.6) the AP·C base pair is predominantly wobble. This is the first report on use of a site specifically N-15 enriched oligonucleotide as a probe of aberrant base pairing in DNA.

Original language	English (US)
Pages (from-to)	89-92
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	165
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(89)91037-1
State	Published - Nov 30 1989
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Characterization of the high pH wobble structure of the 2-aminopurine·cytosine mismatch by N-15 NMR spectroscopy",

abstract = "Transition mutations induced by the base analogue 2-aminopurine arise via the formation of AP·C base pairs during DNA replication. We report here the results of N-15 NMR studies on a duplex oligonucleotide containing N-15 enriched AP and C residues. At high pH (8.6) the AP·C base pair is predominantly wobble. This is the first report on use of a site specifically N-15 enriched oligonucleotide as a probe of aberrant base pairing in DNA.",

author = "Sowers, {Lawrence C.} and Ramon Eritja and Chen, {Fu Ming} and Tasneem Khwaja and Kaplan, {Bruce E.} and Goodman, {Myron F.} and {Victor Fazakerley}, G.",

note = "Funding Information: This research was supported by National Institutes of Health",

year = "1989",

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doi = "10.1016/0006-291X(89)91037-1",

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T1 - Characterization of the high pH wobble structure of the 2-aminopurine·cytosine mismatch by N-15 NMR spectroscopy

AU - Sowers, Lawrence C.

AU - Eritja, Ramon

AU - Chen, Fu Ming

AU - Khwaja, Tasneem

AU - Kaplan, Bruce E.

AU - Goodman, Myron F.

AU - Victor Fazakerley, G.

N1 - Funding Information: This research was supported by National Institutes of Health

PY - 1989/11/30

Y1 - 1989/11/30

N2 - Transition mutations induced by the base analogue 2-aminopurine arise via the formation of AP·C base pairs during DNA replication. We report here the results of N-15 NMR studies on a duplex oligonucleotide containing N-15 enriched AP and C residues. At high pH (8.6) the AP·C base pair is predominantly wobble. This is the first report on use of a site specifically N-15 enriched oligonucleotide as a probe of aberrant base pairing in DNA.

AB - Transition mutations induced by the base analogue 2-aminopurine arise via the formation of AP·C base pairs during DNA replication. We report here the results of N-15 NMR studies on a duplex oligonucleotide containing N-15 enriched AP and C residues. At high pH (8.6) the AP·C base pair is predominantly wobble. This is the first report on use of a site specifically N-15 enriched oligonucleotide as a probe of aberrant base pairing in DNA.

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Characterization of the high pH wobble structure of the 2-aminopurine·cytosine mismatch by N-15 NMR spectroscopy

Abstract

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