Characterization of Culex Flavivirus (Flaviviridae) strains isolated from mosquitoes in the United States and Trinidad

Dal Y. Kim; Hilda Guzman; Rudy Bueno; James A. Dennett; Albert J. Auguste; Christine V F Carrington; Vsevolod L. Popov; Scott C. Weaver; David W C Beasley; Robert B. Tesh

doi:10.1016/j.virol.2008.12.034

Characterization of Culex Flavivirus (Flaviviridae) strains isolated from mosquitoes in the United States and Trinidad

Dal Y. Kim, Hilda Guzman, Rudy Bueno, James A. Dennett, Albert J. Auguste, Christine V F Carrington, Vsevolod L. Popov, Scott C. Weaver, David W C Beasley, Robert B. Tesh

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

Recent reports indicate that flaviviruses similar to the cell fusing agent virus (CFAV) naturally infect a wide variety of mosquito species. These newly recognized insect-specific viruses comprise a distinct CFAV complex within the genus Flavivirus. Here, we describe the isolation and characterization of nine strains of Culex flavivirus (Cx FV), a member of the CFAV complex, from mosquitoes collected in the United States (East Texas) and Trinidad. Phylogenetic analyses of the envelope protein gene sequences of these nine mosquito isolates with those of other CFAV complex flaviviruses in GenBank indicate that the U.S. isolates group with CxFV isolates from Asia (Japan and Indonesia), while the Trinidad isolates are more similar to CxFV isolates from Central America. A discussion follows on the possible biological significance of the CFAV complex flaviviruses.

Original language	English (US)
Pages (from-to)	154-159
Number of pages	6
Journal	Virology
Volume	386
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2008.12.034
State	Published - Mar 30 2009

Keywords

Cell fusing agent virus
Culex flavivirus
Culex quinquefasciatus
Flavivirus
Flavivirus phylogenetics
Mosquito viruses

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2008.12.034

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title = "Characterization of Culex Flavivirus (Flaviviridae) strains isolated from mosquitoes in the United States and Trinidad",

abstract = "Recent reports indicate that flaviviruses similar to the cell fusing agent virus (CFAV) naturally infect a wide variety of mosquito species. These newly recognized insect-specific viruses comprise a distinct CFAV complex within the genus Flavivirus. Here, we describe the isolation and characterization of nine strains of Culex flavivirus (Cx FV), a member of the CFAV complex, from mosquitoes collected in the United States (East Texas) and Trinidad. Phylogenetic analyses of the envelope protein gene sequences of these nine mosquito isolates with those of other CFAV complex flaviviruses in GenBank indicate that the U.S. isolates group with CxFV isolates from Asia (Japan and Indonesia), while the Trinidad isolates are more similar to CxFV isolates from Central America. A discussion follows on the possible biological significance of the CFAV complex flaviviruses.",

keywords = "Cell fusing agent virus, Culex flavivirus, Culex quinquefasciatus, Flavivirus, Flavivirus phylogenetics, Mosquito viruses",

author = "Kim, {Dal Y.} and Hilda Guzman and Rudy Bueno and Dennett, {James A.} and Auguste, {Albert J.} and Carrington, {Christine V F} and Popov, {Vsevolod L.} and Weaver, {Scott C.} and Beasley, {David W C} and Tesh, {Robert B.}",

note = "Funding Information: This work was supported by contracts NO1-AI25489 and NO1-AI30027 from the U.S. National Institutes of Health, by the Trinidad and Tobago Government Research Fund, and by the Caribbean Health Research Council. The authors also thank Maricela Ramos for technical assistance and Dora Salinas for help in preparing the manuscript. ",

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AU - Kim, Dal Y.

AU - Guzman, Hilda

AU - Bueno, Rudy

AU - Dennett, James A.

AU - Auguste, Albert J.

AU - Carrington, Christine V F

AU - Popov, Vsevolod L.

AU - Weaver, Scott C.

AU - Beasley, David W C

AU - Tesh, Robert B.

N1 - Funding Information: This work was supported by contracts NO1-AI25489 and NO1-AI30027 from the U.S. National Institutes of Health, by the Trinidad and Tobago Government Research Fund, and by the Caribbean Health Research Council. The authors also thank Maricela Ramos for technical assistance and Dora Salinas for help in preparing the manuscript.

PY - 2009/3/30

Y1 - 2009/3/30

N2 - Recent reports indicate that flaviviruses similar to the cell fusing agent virus (CFAV) naturally infect a wide variety of mosquito species. These newly recognized insect-specific viruses comprise a distinct CFAV complex within the genus Flavivirus. Here, we describe the isolation and characterization of nine strains of Culex flavivirus (Cx FV), a member of the CFAV complex, from mosquitoes collected in the United States (East Texas) and Trinidad. Phylogenetic analyses of the envelope protein gene sequences of these nine mosquito isolates with those of other CFAV complex flaviviruses in GenBank indicate that the U.S. isolates group with CxFV isolates from Asia (Japan and Indonesia), while the Trinidad isolates are more similar to CxFV isolates from Central America. A discussion follows on the possible biological significance of the CFAV complex flaviviruses.

AB - Recent reports indicate that flaviviruses similar to the cell fusing agent virus (CFAV) naturally infect a wide variety of mosquito species. These newly recognized insect-specific viruses comprise a distinct CFAV complex within the genus Flavivirus. Here, we describe the isolation and characterization of nine strains of Culex flavivirus (Cx FV), a member of the CFAV complex, from mosquitoes collected in the United States (East Texas) and Trinidad. Phylogenetic analyses of the envelope protein gene sequences of these nine mosquito isolates with those of other CFAV complex flaviviruses in GenBank indicate that the U.S. isolates group with CxFV isolates from Asia (Japan and Indonesia), while the Trinidad isolates are more similar to CxFV isolates from Central America. A discussion follows on the possible biological significance of the CFAV complex flaviviruses.

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KW - Flavivirus phylogenetics

KW - Mosquito viruses

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Characterization of Culex Flavivirus (Flaviviridae) strains isolated from mosquitoes in the United States and Trinidad

Abstract

Keywords

ASJC Scopus subject areas

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