Characterization of an alloherpesvirus from wild lake sturgeon Acipenser fulvescens in Wisconsin (USA)

Logan Walker; Kuttichantran Subramaniam; Pedro H.O. Viadanna; Jordan A. Vann; Susan Marcquenski; Danielle Godard; Elizabeth Kieran; Salvatore Frasca; Vsevolod L. Popov; Karen Kerr; Andrew J. Davison; Thomas B. Waltzek

doi:10.3354/DAO03661

Characterization of an alloherpesvirus from wild lake sturgeon Acipenser fulvescens in Wisconsin (USA)

Logan Walker, Kuttichantran Subramaniam, Pedro H.O. Viadanna, Jordan A. Vann, Susan Marcquenski, Danielle Godard, Elizabeth Kieran, Salvatore Frasca, Vsevolod L. Popov, Karen Kerr, Andrew J. Davison, Thomas B. Waltzek

Pathology

Research output: Contribution to journal › Article › peer-review

Abstract

In the spring of 2017, 2 adult lake sturgeon (LS) Acipenser fulvescens captured from the Wolf River, Wisconsin (USA), presented with multiple cutaneous plaques that, upon microscopic examination, indicated proliferative epidermitis. Ultrastructural examination of affected keratinocytes revealed particles in the nucleus having a morphology typical of herpesviruses. A degenerate PCR assay targeting the DNA polymerase catalytic subunit (pol) gene of large double-stranded DNA viruses generated amplicons of the anticipated size from skin samples, and sequences of amplicons confirmed the presence of a novel alloherpesvirus (lake sturgeon herpesvirus, LSHV) related to acipenserid herpesvirus 1 (AciHV1). The complete genome (202 660 bp) of this virus was sequenced using a MiSeq System, and phylogenetic analyses substantiated the close relationship to AciHV1. A PCR assay targeting the LSHV DNA packaging terminase subunit 1 (ter1) gene demonstrated the presence of the virus in 39/42 skin lesion samples collected from wild LS captured in 2017−2019 and 2021 in 4/4 rivers in Wisconsin. Future efforts to isolate LSHV in cell culture would facilitate challenge studies to determine the disease potential of the virus.

Original language	English (US)
Pages (from-to)	83-96
Number of pages	14
Journal	Diseases of Aquatic Organisms
Volume	149
DOIs	https://doi.org/10.3354/DAO03661
State	Published - 2022

Keywords

Acipenserid herpesvirus 1
Alloherpesviridae
Hyperplastic dermatitis
Lake sturgeon
Lake sturgeon herpesvirus
Phylogenetics

ASJC Scopus subject areas

General Medicine

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10.3354/DAO03661

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@article{e8389370d60b4cd59f20482f1d6c221f,

title = "Characterization of an alloherpesvirus from wild lake sturgeon Acipenser fulvescens in Wisconsin (USA)",

abstract = "In the spring of 2017, 2 adult lake sturgeon (LS) Acipenser fulvescens captured from the Wolf River, Wisconsin (USA), presented with multiple cutaneous plaques that, upon microscopic examination, indicated proliferative epidermitis. Ultrastructural examination of affected keratinocytes revealed particles in the nucleus having a morphology typical of herpesviruses. A degenerate PCR assay targeting the DNA polymerase catalytic subunit (pol) gene of large double-stranded DNA viruses generated amplicons of the anticipated size from skin samples, and sequences of amplicons confirmed the presence of a novel alloherpesvirus (lake sturgeon herpesvirus, LSHV) related to acipenserid herpesvirus 1 (AciHV1). The complete genome (202 660 bp) of this virus was sequenced using a MiSeq System, and phylogenetic analyses substantiated the close relationship to AciHV1. A PCR assay targeting the LSHV DNA packaging terminase subunit 1 (ter1) gene demonstrated the presence of the virus in 39/42 skin lesion samples collected from wild LS captured in 2017−2019 and 2021 in 4/4 rivers in Wisconsin. Future efforts to isolate LSHV in cell culture would facilitate challenge studies to determine the disease potential of the virus.",

keywords = "Acipenserid herpesvirus 1, Alloherpesviridae, Hyperplastic dermatitis, Lake sturgeon, Lake sturgeon herpesvirus, Phylogenetics",

author = "Logan Walker and Kuttichantran Subramaniam and Viadanna, {Pedro H.O.} and Vann, {Jordan A.} and Susan Marcquenski and Danielle Godard and Elizabeth Kieran and Salvatore Frasca and Popov, {Vsevolod L.} and Karen Kerr and Davison, {Andrew J.} and Waltzek, {Thomas B.}",

note = "Publisher Copyright: {\textcopyright} The authors 2022. Open Access under Creative Commons by Attribution Licence. Use, distribution and reproduction are unrestricted. Authors and original publication must be credited.",

year = "2022",

doi = "10.3354/DAO03661",

language = "English (US)",

volume = "149",

pages = "83--96",

journal = "Diseases of Aquatic Organisms",

issn = "0177-5103",

publisher = "Inter-Research",

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TY - JOUR

T1 - Characterization of an alloherpesvirus from wild lake sturgeon Acipenser fulvescens in Wisconsin (USA)

AU - Walker, Logan

AU - Subramaniam, Kuttichantran

AU - Viadanna, Pedro H.O.

AU - Vann, Jordan A.

AU - Marcquenski, Susan

AU - Godard, Danielle

AU - Kieran, Elizabeth

AU - Frasca, Salvatore

AU - Popov, Vsevolod L.

AU - Kerr, Karen

AU - Davison, Andrew J.

AU - Waltzek, Thomas B.

N1 - Publisher Copyright: © The authors 2022. Open Access under Creative Commons by Attribution Licence. Use, distribution and reproduction are unrestricted. Authors and original publication must be credited.

PY - 2022

Y1 - 2022

N2 - In the spring of 2017, 2 adult lake sturgeon (LS) Acipenser fulvescens captured from the Wolf River, Wisconsin (USA), presented with multiple cutaneous plaques that, upon microscopic examination, indicated proliferative epidermitis. Ultrastructural examination of affected keratinocytes revealed particles in the nucleus having a morphology typical of herpesviruses. A degenerate PCR assay targeting the DNA polymerase catalytic subunit (pol) gene of large double-stranded DNA viruses generated amplicons of the anticipated size from skin samples, and sequences of amplicons confirmed the presence of a novel alloherpesvirus (lake sturgeon herpesvirus, LSHV) related to acipenserid herpesvirus 1 (AciHV1). The complete genome (202 660 bp) of this virus was sequenced using a MiSeq System, and phylogenetic analyses substantiated the close relationship to AciHV1. A PCR assay targeting the LSHV DNA packaging terminase subunit 1 (ter1) gene demonstrated the presence of the virus in 39/42 skin lesion samples collected from wild LS captured in 2017−2019 and 2021 in 4/4 rivers in Wisconsin. Future efforts to isolate LSHV in cell culture would facilitate challenge studies to determine the disease potential of the virus.

AB - In the spring of 2017, 2 adult lake sturgeon (LS) Acipenser fulvescens captured from the Wolf River, Wisconsin (USA), presented with multiple cutaneous plaques that, upon microscopic examination, indicated proliferative epidermitis. Ultrastructural examination of affected keratinocytes revealed particles in the nucleus having a morphology typical of herpesviruses. A degenerate PCR assay targeting the DNA polymerase catalytic subunit (pol) gene of large double-stranded DNA viruses generated amplicons of the anticipated size from skin samples, and sequences of amplicons confirmed the presence of a novel alloherpesvirus (lake sturgeon herpesvirus, LSHV) related to acipenserid herpesvirus 1 (AciHV1). The complete genome (202 660 bp) of this virus was sequenced using a MiSeq System, and phylogenetic analyses substantiated the close relationship to AciHV1. A PCR assay targeting the LSHV DNA packaging terminase subunit 1 (ter1) gene demonstrated the presence of the virus in 39/42 skin lesion samples collected from wild LS captured in 2017−2019 and 2021 in 4/4 rivers in Wisconsin. Future efforts to isolate LSHV in cell culture would facilitate challenge studies to determine the disease potential of the virus.

KW - Acipenserid herpesvirus 1

KW - Alloherpesviridae

KW - Hyperplastic dermatitis

KW - Lake sturgeon

KW - Lake sturgeon herpesvirus

KW - Phylogenetics

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AN - SCOPUS:85131707518

SN - 0177-5103

VL - 149

SP - 83

EP - 96

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

ER -

Characterization of an alloherpesvirus from wild lake sturgeon Acipenser fulvescens in Wisconsin (USA)

Abstract

Keywords

ASJC Scopus subject areas

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