Characterization of a murine model of fetal programming of atherosclerosis

Nima Goharkhay; Elena Sbrana; Phyllis K. Gamble; Esther H. Tamayo; Ancizar Betancourt; Karina Villarreal; Gary D.V. Hankins; George R. Saade; Monica Longo

doi:10.1016/j.ajog.2007.08.002

Characterization of a murine model of fetal programming of atherosclerosis

Nima Goharkhay, Elena Sbrana, Phyllis K. Gamble, Esther H. Tamayo, Ancizar Betancourt, Karina Villarreal, Gary D.V. Hankins, George R. Saade, Monica Longo

Obstetrics & Gynecology

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Objective: The objective of the study was to investigate the effect of fetal programming on the development of atherosclerosis in the offspring in a mouse model. Study Design: Male and female mice of the wild type and the knockout for the apoprotein E (apoE) gene were cross-bred to obtain all 4 possible genetic offspring types. The offspring were kept on regular chow and killed at 8 months of age. Levels of total cholesterol and triglycerides were determined. The aortic arch was examined for the presence and severity of atherosclerosis. Kidney and liver sections were analyzed for pathologic changes. Results: We found increased total cholesterol levels and incidence of atherosclerosis in offspring born to hypercholesterolemic mothers as compared with genomically similar animals born to wild-type mothers. These animals also showed kidney and liver lesions consistent with chronic hypercholesterolemia. Conclusions: There is a strong effect of fetal programming on the development of atherosclerosis in the apoE mouse model.

Original language	English (US)
Pages (from-to)	416.e1-416.e5
Journal	American journal of obstetrics and gynecology
Volume	197
Issue number	4
DOIs	https://doi.org/10.1016/j.ajog.2007.08.002
State	Published - Oct 2007

Keywords

atherosclerosis
fetal development
fetal programming
hypercholesterolemia
mouse model

ASJC Scopus subject areas

Obstetrics and Gynecology

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10.1016/j.ajog.2007.08.002

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title = "Characterization of a murine model of fetal programming of atherosclerosis",

abstract = "Objective: The objective of the study was to investigate the effect of fetal programming on the development of atherosclerosis in the offspring in a mouse model. Study Design: Male and female mice of the wild type and the knockout for the apoprotein E (apoE) gene were cross-bred to obtain all 4 possible genetic offspring types. The offspring were kept on regular chow and killed at 8 months of age. Levels of total cholesterol and triglycerides were determined. The aortic arch was examined for the presence and severity of atherosclerosis. Kidney and liver sections were analyzed for pathologic changes. Results: We found increased total cholesterol levels and incidence of atherosclerosis in offspring born to hypercholesterolemic mothers as compared with genomically similar animals born to wild-type mothers. These animals also showed kidney and liver lesions consistent with chronic hypercholesterolemia. Conclusions: There is a strong effect of fetal programming on the development of atherosclerosis in the apoE mouse model.",

keywords = "atherosclerosis, fetal development, fetal programming, hypercholesterolemia, mouse model",

author = "Nima Goharkhay and Elena Sbrana and Gamble, {Phyllis K.} and Tamayo, {Esther H.} and Ancizar Betancourt and Karina Villarreal and Hankins, {Gary D.V.} and Saade, {George R.} and Monica Longo",

year = "2007",

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doi = "10.1016/j.ajog.2007.08.002",

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volume = "197",

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AU - Goharkhay, Nima

AU - Sbrana, Elena

AU - Gamble, Phyllis K.

AU - Tamayo, Esther H.

AU - Betancourt, Ancizar

AU - Villarreal, Karina

AU - Hankins, Gary D.V.

AU - Saade, George R.

AU - Longo, Monica

PY - 2007/10

Y1 - 2007/10

N2 - Objective: The objective of the study was to investigate the effect of fetal programming on the development of atherosclerosis in the offspring in a mouse model. Study Design: Male and female mice of the wild type and the knockout for the apoprotein E (apoE) gene were cross-bred to obtain all 4 possible genetic offspring types. The offspring were kept on regular chow and killed at 8 months of age. Levels of total cholesterol and triglycerides were determined. The aortic arch was examined for the presence and severity of atherosclerosis. Kidney and liver sections were analyzed for pathologic changes. Results: We found increased total cholesterol levels and incidence of atherosclerosis in offspring born to hypercholesterolemic mothers as compared with genomically similar animals born to wild-type mothers. These animals also showed kidney and liver lesions consistent with chronic hypercholesterolemia. Conclusions: There is a strong effect of fetal programming on the development of atherosclerosis in the apoE mouse model.

AB - Objective: The objective of the study was to investigate the effect of fetal programming on the development of atherosclerosis in the offspring in a mouse model. Study Design: Male and female mice of the wild type and the knockout for the apoprotein E (apoE) gene were cross-bred to obtain all 4 possible genetic offspring types. The offspring were kept on regular chow and killed at 8 months of age. Levels of total cholesterol and triglycerides were determined. The aortic arch was examined for the presence and severity of atherosclerosis. Kidney and liver sections were analyzed for pathologic changes. Results: We found increased total cholesterol levels and incidence of atherosclerosis in offspring born to hypercholesterolemic mothers as compared with genomically similar animals born to wild-type mothers. These animals also showed kidney and liver lesions consistent with chronic hypercholesterolemia. Conclusions: There is a strong effect of fetal programming on the development of atherosclerosis in the apoE mouse model.

KW - atherosclerosis

KW - fetal development

KW - fetal programming

KW - hypercholesterolemia

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ER -

Characterization of a murine model of fetal programming of atherosclerosis

Abstract

Keywords

ASJC Scopus subject areas

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