CD177^＋中性粒细胞在炎症性肠病的表达及效应初步研究

Objective: To examine CD177 expression in peripheral blood and inflamed mucosa from active patients with inflammatory bowel disease (IBD), and explore its potential role in regulating the migration of neutrophils. Methods: Peripheral blood and inflamed intestinal mucosa were harvested from patients with active Crohn′s disease (n=18), active ulcerative colitis (n=21) and healthy controls (n=14). CD177 expression was determined by flow cytometry. CD177⁺ and CD177^- neutrophils were also isolated from peripheral blood of patients with active IBD by immunomagnetic beads, and the migration of these neutrophils was analyzed by Transwell. Results: CD177 expression was highly increased in peripheral blood neutrophils and inflamed mucosa from patients with active IBD compared with that in healthy controls (P<0.05). Purified CD177⁺ and CD177^- neutrophils were isolated from active IBD patients, and a Transwell analysis revealed that the migration capacity of CD177⁺ neutrophils was markedly increased than that of CD177^- neutrophils in all groups (P<0.05). Moreover, the migration capacity of CD177⁺ neutrophils from IBD patients was also markedly increased compared with that from healthy controls (P<0.05). Conclusion: Our data demonstrate that CD177 expression is increased in patients with IBD, and that CD177 promotes neutrophil migration and homing into intestinal mucosa. CD177⁺ neutrophils may play an important regulatory role in modulating intestinal mucosal inflammation.