Abstract
We examined the effect of cationic lipid-mediated gene transfection of nerve growth factor (NGF) in primary septo-hippocampal cell cultures. Rat NGF cDNA was subcloned into a pUC19-based plasmid containing a CMV promoter. Two days after NGF gene transfection in primary cell cultures, ELISA confirmed increases in NGF protein secretion from transfected cells. To study the biological effect of cationic lipid-mediated NGF gene transfection, we analyzed the amount of neurofilament protein from NGF-transfected cell cultures. Western blot and immunohistochemical analyses detected significant increases in the phosphorylated form of neurofilament proteins in the cultures after cationic lipid-mediated NGF cDNA transfection. Cationic lipid-mediated NGF cDNA transfection aid not cause significant changes in the total amount of neurofilament protein. Our studies suggest that cationic lipid-mediated NGF gene transfection can increase neurofilament phosphorylation but not total neurofilament protein.
Original language | English (US) |
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Pages (from-to) | 13-20 |
Number of pages | 8 |
Journal | Molecular Brain Research |
Volume | 43 |
Issue number | 1-2 |
DOIs | |
State | Published - Dec 31 1996 |
Keywords
- Cationic lipid
- Cell culture
- Gene transfer
- Nerve growth factor
- Neurofilament
- Septo-hippocampal system
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience