TY - JOUR
T1 - Caffeinated coffee blunts the myocardial protective effects of statins against ischemia-reperfusion injury in the rat
AU - Ye, Yumei
AU - Abu Said, Ghassan H.
AU - Lin, Yu
AU - Manickavasagam, Saraswathy
AU - Hughes, Michael G.
AU - McAdoo, David J.
AU - Perez-Polo, Regino J.
AU - Birnbaum, Yochai
PY - 2008/8
Y1 - 2008/8
N2 - Purpose: We asked whether caffeinated coffee (CC) blunts the infarct size (IS)-limiting effects of atorvastatin (ATV). Background: Adenosine receptor activation is essential for mediating the IS-limiting effects of statins. Caffeine is a nonspecific adenosine receptor blocker, and thus drinking CC may block the myocardial protective effects of statins. Methods: Rat received 3-day ATV (10 mg/kg/day) or water by oral gavage once daily. Drinking water was replaced by water + sugar (7.5 g/100 ml), CC with sugar, or decaffeinated coffee (DC) with sugar. On the 4th day, rats were anesthetized and underwent 30 min of coronary artery occlusion and 4 h reperfusion. Area at risk was assessed by blue dye and infarct size by TTC. Results: Body weight and area at risk was comparable among groups. IS was 25.1±3.9% of the area at risk in the control group. In rats not receiving ATV, CC (25.5±3.1%) and DC (34.0±2.8%) did not affect IS. IS was significantly reduced by ATV in the water + sugar (11.7±0.7%, p=0.015) and DC (11.5±1.0%; p<0.001) groups, but not in the CC group (32.3±3.0%; p=0.719). ATV increased myocardial levels of Ser-473 phosphorylated Akt in the water + sugar and DC groups, but not in the CC group. Conclusions: CC, but not DC, abrogated the IS-limiting effects of ATV by blocking the adenosine receptors and preventing the phosphorylation of Akt. CC did not affect IS in rats not receiving ATV.
AB - Purpose: We asked whether caffeinated coffee (CC) blunts the infarct size (IS)-limiting effects of atorvastatin (ATV). Background: Adenosine receptor activation is essential for mediating the IS-limiting effects of statins. Caffeine is a nonspecific adenosine receptor blocker, and thus drinking CC may block the myocardial protective effects of statins. Methods: Rat received 3-day ATV (10 mg/kg/day) or water by oral gavage once daily. Drinking water was replaced by water + sugar (7.5 g/100 ml), CC with sugar, or decaffeinated coffee (DC) with sugar. On the 4th day, rats were anesthetized and underwent 30 min of coronary artery occlusion and 4 h reperfusion. Area at risk was assessed by blue dye and infarct size by TTC. Results: Body weight and area at risk was comparable among groups. IS was 25.1±3.9% of the area at risk in the control group. In rats not receiving ATV, CC (25.5±3.1%) and DC (34.0±2.8%) did not affect IS. IS was significantly reduced by ATV in the water + sugar (11.7±0.7%, p=0.015) and DC (11.5±1.0%; p<0.001) groups, but not in the CC group (32.3±3.0%; p=0.719). ATV increased myocardial levels of Ser-473 phosphorylated Akt in the water + sugar and DC groups, but not in the CC group. Conclusions: CC, but not DC, abrogated the IS-limiting effects of ATV by blocking the adenosine receptors and preventing the phosphorylation of Akt. CC did not affect IS in rats not receiving ATV.
KW - Adenosine
KW - Atorvastatin
KW - Caffeine
KW - Coffee
KW - Infarct size
UR - http://www.scopus.com/inward/record.url?scp=48349147462&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=48349147462&partnerID=8YFLogxK
U2 - 10.1007/s10557-008-6105-z
DO - 10.1007/s10557-008-6105-z
M3 - Article
C2 - 18327703
AN - SCOPUS:48349147462
SN - 0920-3206
VL - 22
SP - 275
EP - 282
JO - Cardiovascular Drugs and Therapy
JF - Cardiovascular Drugs and Therapy
IS - 4
ER -