Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherin

Rodrigo I. Santos; Philipp A. Ilinykh; Colette A. Pietzsch; Adam Ronk; Kai Huang; Natalia A. Kuzmina; Fuchun Zhou; James E. Crowe; Alexander Bukreyev

doi:10.1016/j.celrep.2023.113254

Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherin

Rodrigo I. Santos, Philipp A. Ilinykh, Colette A. Pietzsch, Adam Ronk, Kai Huang, Natalia A. Kuzmina, Fuchun Zhou, James E. Crowe, Alexander Bukreyev

Research output: Contribution to journal › Article › peer-review

Abstract

Ebola virus (EBOV) and Bundibugyo virus (BDBV) belong to the family Filoviridae and cause a severe disease in humans. We previously isolated a large panel of monoclonal antibodies from B cells of human survivors from the 2007 Uganda BDBV outbreak, 16 survivors from the 2014 EBOV outbreak in the Democratic Republic of the Congo, and one survivor from the West African 2013–2016 EBOV epidemic. Here, we demonstrate that EBOV and BDBV are capable of spreading to neighboring cells through intercellular connections in a process that depends upon actin and T cell immunoglobulin and mucin 1 protein. We quantify spread through intercellular connections by immunofluorescence microscopy and flow cytometry. One of the antibodies, BDBV223, specific to the membrane-proximal external region, induces virus accumulation at the plasma membrane. The inhibiting activity of BDBV223 depends on BST2/tetherin.

Original language	English (US)
Article number	113254
Journal	Cell Reports
Volume	42
Issue number	10
DOIs	https://doi.org/10.1016/j.celrep.2023.113254
State	Published - Oct 31 2023
Externally published	Yes

Keywords

BST2
CP: Immunology
CP: Microbiology
Ebola virus
MPER
TIM-1
cell-to-cell transmission
intercellular connections
monoclonal antibodies
tetherin
virus egress

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.celrep.2023.113254

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title = "Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherin",

abstract = "Ebola virus (EBOV) and Bundibugyo virus (BDBV) belong to the family Filoviridae and cause a severe disease in humans. We previously isolated a large panel of monoclonal antibodies from B cells of human survivors from the 2007 Uganda BDBV outbreak, 16 survivors from the 2014 EBOV outbreak in the Democratic Republic of the Congo, and one survivor from the West African 2013–2016 EBOV epidemic. Here, we demonstrate that EBOV and BDBV are capable of spreading to neighboring cells through intercellular connections in a process that depends upon actin and T cell immunoglobulin and mucin 1 protein. We quantify spread through intercellular connections by immunofluorescence microscopy and flow cytometry. One of the antibodies, BDBV223, specific to the membrane-proximal external region, induces virus accumulation at the plasma membrane. The inhibiting activity of BDBV223 depends on BST2/tetherin.",

keywords = "BST2, CP: Immunology, CP: Microbiology, Ebola virus, MPER, TIM-1, cell-to-cell transmission, intercellular connections, monoclonal antibodies, tetherin, virus egress",

author = "Santos, {Rodrigo I.} and Ilinykh, {Philipp A.} and Pietzsch, {Colette A.} and Adam Ronk and Kai Huang and Kuzmina, {Natalia A.} and Fuchun Zhou and Crowe, {James E.} and Alexander Bukreyev",

note = "Publisher Copyright: {\textcopyright} 2023 The Authors",

year = "2023",

month = oct,

day = "31",

doi = "10.1016/j.celrep.2023.113254",

language = "English (US)",

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T1 - Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherin

AU - Santos, Rodrigo I.

AU - Ilinykh, Philipp A.

AU - Pietzsch, Colette A.

AU - Ronk, Adam

AU - Huang, Kai

AU - Kuzmina, Natalia A.

AU - Zhou, Fuchun

AU - Crowe, James E.

AU - Bukreyev, Alexander

PY - 2023/10/31

Y1 - 2023/10/31

N2 - Ebola virus (EBOV) and Bundibugyo virus (BDBV) belong to the family Filoviridae and cause a severe disease in humans. We previously isolated a large panel of monoclonal antibodies from B cells of human survivors from the 2007 Uganda BDBV outbreak, 16 survivors from the 2014 EBOV outbreak in the Democratic Republic of the Congo, and one survivor from the West African 2013–2016 EBOV epidemic. Here, we demonstrate that EBOV and BDBV are capable of spreading to neighboring cells through intercellular connections in a process that depends upon actin and T cell immunoglobulin and mucin 1 protein. We quantify spread through intercellular connections by immunofluorescence microscopy and flow cytometry. One of the antibodies, BDBV223, specific to the membrane-proximal external region, induces virus accumulation at the plasma membrane. The inhibiting activity of BDBV223 depends on BST2/tetherin.

AB - Ebola virus (EBOV) and Bundibugyo virus (BDBV) belong to the family Filoviridae and cause a severe disease in humans. We previously isolated a large panel of monoclonal antibodies from B cells of human survivors from the 2007 Uganda BDBV outbreak, 16 survivors from the 2014 EBOV outbreak in the Democratic Republic of the Congo, and one survivor from the West African 2013–2016 EBOV epidemic. Here, we demonstrate that EBOV and BDBV are capable of spreading to neighboring cells through intercellular connections in a process that depends upon actin and T cell immunoglobulin and mucin 1 protein. We quantify spread through intercellular connections by immunofluorescence microscopy and flow cytometry. One of the antibodies, BDBV223, specific to the membrane-proximal external region, induces virus accumulation at the plasma membrane. The inhibiting activity of BDBV223 depends on BST2/tetherin.

KW - BST2

KW - CP: Immunology

KW - CP: Microbiology

KW - Ebola virus

KW - MPER

KW - TIM-1

KW - cell-to-cell transmission

KW - intercellular connections

KW - monoclonal antibodies

KW - tetherin

KW - virus egress

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DO - 10.1016/j.celrep.2023.113254

M3 - Article

C2 - 37858466

AN - SCOPUS:85174140968

SN - 2211-1247

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JO - Cell Reports

JF - Cell Reports

IS - 10

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Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherin

Abstract

Keywords

ASJC Scopus subject areas

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